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Immunization of BALB/c mice with sonicates of M. smegmatis and kansasii and fusion of splenocytes of the immunized mice with cells of syngeneic myeloma P3X63Ag8653 yielded hybrid clones synthetizing antibodies to these antigens. The selection of hybrid clones and analysis of the antibodies specificity were performed at ELISA using the immunization antigens and antigens from other mycobacteria and E. coli. To define immunoglobulin class of the antibodies the authors used antiglobulin sera of class A, M, G. The monoclonal antibodies belonged to IgG. Molecular mass of polypeptides carrying the epitope against which the antibodies were directed was measured at immunoblotting. Two antibodies reacted only with M. smegmatis, 38 and 10 kD proteins. The other 2 cross-reacted with other mycobacteria and were directed against epitopes on polypeptides varying in molecular mass.
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
June 1988,
Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology,
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
January 1987,
Journal of hygiene, epidemiology, microbiology, and immunology,
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
April 1996,
Molecular microbiology,
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
September 1992,
Research in microbiology,
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
April 2001,
Revue scientifique et technique (International Office of Epizootics),
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
January 1973,
Journal of general microbiology,
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
January 1988,
Voprosy virusologii,
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
January 1971,
Bollettino dell'Istituto sieroterapico milanese,
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
December 1984,
Clinical and experimental immunology,
N V Kulikovskaia, and
M A Kapina, and
O A Kalinina, and
L N Chernousova
September 2021,
Annals of laboratory medicine,
