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A method for calculating radioimmunoassay standard curves, based on the theory of Ekins et al., is described. Because a four-parameter model is used, nonlinear standard curves are the result. The calibration curve is fitted to the measured standard points by means of a weighted least-squares method. The program based on this model can be easily processed on a desk-top calculator. For all 250 runs of six different assays, very good standard curves could be obtained. The mean deviation between the concentrations of the standard points and the corresponding calculated values was about 6%. In 26% of the cases it could be shown that the model we describe gave significantly better results than did two simpler ones.
A J Naus, and
P S Kuppens, and
A Borst
February 1980,
Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie,
A J Naus, and
P S Kuppens, and
A Borst
May 1974,
Journal of immunological methods,
A J Naus, and
P S Kuppens, and
A Borst
January 1978,
Gynecologic investigation,
A J Naus, and
P S Kuppens, and
A Borst
July 1973,
Clinical chemistry,
A J Naus, and
P S Kuppens, and
A Borst
February 1974,
Computers and biomedical research, an international journal,
A J Naus, and
P S Kuppens, and
A Borst
January 1982,
Gynecologic and obstetric investigation,
A J Naus, and
P S Kuppens, and
A Borst
October 1983,
Clinical chemistry,
A J Naus, and
P S Kuppens, and
A Borst
September 1976,
Steroids,
A J Naus, and
P S Kuppens, and
A Borst
March 1977,
Steroids,
A J Naus, and
P S Kuppens, and
A Borst
November 1969,
Archiv der Pharmazie und Berichte der Deutschen Pharmazeutischen Gesellschaft,
