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We have made several improvements in the method of fixation of the inner ear and the enzyme-histo-chemical technique for carbonic anhydrase (CA) detection. The results confirmed that CA is localized in the hair cells of the organ of Corti, Deiters' cells or nerve endings, inner pillar cells, Boettcher's cells, stria vascularis, spiral ligament, spiral limbus, and spiral ganglion cells. These results generally agree with previous histochemical observations but showed some differences. Our method preserved tissue morphology and showed more detailed localization of CA activity in the inner ear. In particular, the marginal zone of stria vascularis and the epithelial cells of spiral prominence, facing the endolymph, showed no CA activity, while the suprastrial region of the spiral ligament and the supralimbal region of the spiral limbus, juxtaposed to the perilymph, showed CA activity. In outer hair cells, the cuticular plate, which faces the endolymph showed CA activity, but the lateral membrane, which faces the perilymph showed no CA activity. In contrast, the inner hair cell cytoplasm showed diffuse CA activity. These results will be useful in considering ion exchange between endolymph and its adjacent cells, and between perilymph and its adjacent structures.
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
January 1983,
American journal of otolaryngology,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
January 1968,
Archiv fur klinische und experimentelle Ohren- Nasen- und Kehlkopfheilkunde,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
March 1977,
Proceedings of the National Academy of Sciences of the United States of America,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
April 1966,
Zeitschrift fur Laryngologie, Rhinologie, Otologie und ihre Grenzgebiete,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
January 1971,
Archiv fur klinische und experimentelle Ohren- Nasen- und Kehlkopfheilkunde,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
February 1961,
Nature,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
January 1991,
Acta oto-laryngologica,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
January 1985,
Acta oto-laryngologica. Supplementum,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
July 1985,
Stain technology,
H O Okamura, and
N Sugai, and
K Suzuki, and
I Ohtani
March 1994,
Acta oto-laryngologica,
