Biomaterial Database

Recognition of aminoglycoside antibiotics by enterococcal-staphylococcal aminoglycoside 3'-phosphotransferase type IIIa: role of substrate amino groups. 1996

G A McKay, and J Roestamadji, and S Mobashery, and G D Wright

Department of Biochemistry, McMaster University, Hamilton, Ontario.

The interactions of the aminoglycoside 3'-phosphotransferase IIIa with aminoglycoside antibiotics lacking specific amino groups were examined by steady-state kinetic analyses. The results demonstrate that an amino group on C-1 and either an amino or a hydroxyl group at the 2' and 6' positions are important for detoxification of aminoglycosides by this enzyme.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D000588	Amines	A group of compounds derived from ammonia by substituting organic radicals for the hydrogens. (From Grant & Hackh's Chemical Dictionary, 5th ed)	Amine
D000617	Aminoglycosides	Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS.	Aminoglycoside
D000900	Anti-Bacterial Agents	Substances that inhibit the growth or reproduction of BACTERIA.	Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D013210	Staphylococcus	A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
D016983	Enterococcus	A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.
D017853	Phosphotransferases (Alcohol Group Acceptor)	A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.
D019868	Kanamycin Kinase	A class of enzymes that inactivate aminocyclitol-aminoglycoside antibiotics (AMINOGLYCOSIDES) by regiospecific PHOSPHORYLATION of the 3' and/or 5' hydroxyl.	Aminoglycoside Phosphotransferase,Neomycin Phosphotransferase,APH(3')-IIIa,APH(3')-IIb,APHVII,APT-3'-I and II,Amikacin 3'-Phosphotransferase,Aminocyclitol Phosphotransferase,Aminoglycoside 3'-Phosphotransferase Type VIII,Aminoglycoside 3'-Phosphotransferases (I and II),Aminoglycoside Phosphotransferase Type III,Kanamycin-Neomycin Phosphate Transferase,Neomycin Phosphotransferase II,aphVII Gene Product,3'-Phosphotransferase, Amikacin,APT 3' I and II,Amikacin 3' Phosphotransferase,Aminoglycoside 3' Phosphotransferase Type VIII,Kanamycin Neomycin Phosphate Transferase,Kinase, Kanamycin,Phosphate Transferase, Kanamycin-Neomycin,Phosphotransferase II, Neomycin,Phosphotransferase, Aminocyclitol,Phosphotransferase, Aminoglycoside,Phosphotransferase, Neomycin,Transferase, Kanamycin-Neomycin Phosphate