Biomaterial Database

Development of locustatachykinin immunopositive neurons in the central complex of the beetle Tenebrio molitor. 1996

R Wegerhoff, and O Breidbach, and M Lobemeier

Biochemisches Institut, University of Kiel, Germany. rwegerhoff@biochem.uni-kiel.de

Locustatachykinin-immunoreactive (LomTK-IR) interneurons were found to be associated with the central complex, a prominent neuropil region of the insect brain. The structures and development of this set of brain interneurons was studied from the embryo onward in the beetle Tenebrio molitor, showing individual neurons that persist from the late embryo to the adult stage. Their essential structural characteristics were already present in the late embryo, but distinct parts of their arborization patterns became newly formed throughout development. Using a combination of immunohistochemistry and single-cell injection, we demonstrated minute structural changes, allowing a characterization of structural plasticity of identifiable, persistent, neuropeptidergic neurons throughout ontogenesis. Furthermore, this study has provided new information about basic principles of central brain neuroanatomy and the development of a distinct midbrain region of the insect brain, the central complex. The development of its basic connections, the connections between the fan-shaped body and the protocerebral bridge, and the compartmentation of these neuropil regions were shown, using LomTK-IR neurons as marker structures. These basic features of the central complex-associated LomTK-immunopositive neurons were formed in the embryonic brain, whereas in metamorphosis, reorganization of these persistent interneurons was restricted to the formation of a precisely defined projection of their side branches.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007814	Larva	Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.	Maggots,Tadpoles,Larvae,Maggot,Tadpole
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D011679	Pupa	An inactive stage between the larval and adult stages in the life cycle of INSECTA.	Chrysalis,Pupae
D004625	Embryo, Nonmammalian	The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.	Embryonic Structures, Nonmammalian,Embryo, Non-Mammalian,Embryonic Structures, Non-Mammalian,Nonmammalian Embryo,Nonmammalian Embryo Structures,Nonmammalian Embryonic Structures,Embryo Structure, Nonmammalian,Embryo Structures, Nonmammalian,Embryo, Non Mammalian,Embryonic Structure, Non-Mammalian,Embryonic Structure, Nonmammalian,Embryonic Structures, Non Mammalian,Embryos, Non-Mammalian,Embryos, Nonmammalian,Non-Mammalian Embryo,Non-Mammalian Embryonic Structure,Non-Mammalian Embryonic Structures,Non-Mammalian Embryos,Nonmammalian Embryo Structure,Nonmammalian Embryonic Structure,Nonmammalian Embryos,Structure, Non-Mammalian Embryonic,Structure, Nonmammalian Embryo,Structure, Nonmammalian Embryonic,Structures, Non-Mammalian Embryonic,Structures, Nonmammalian Embryo,Structures, Nonmammalian Embryonic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013712	Tenebrio	A genus of beetles which infests grain products. Its larva is called mealworm.	Mealworm,Mealworms,Tenebrios
D015320	Tachykinins	A family of biologically active peptides sharing a common conserved C-terminal sequence, -Phe-X-Gly-Leu-Met-NH2, where X is either an aromatic or a branched aliphatic amino acid. Members of this family have been found in mammals, amphibians, and mollusks. Tachykinins have diverse pharmacological actions in the central nervous system and the cardiovascular, genitourinary, respiratory, and gastrointestinal systems, as well as in glandular tissues. This diversity of activity is due to the existence of three or more subtypes of tachykinin receptors.	Tachykinin
D019476	Insect Proteins	Proteins found in any species of insect.	Insect Protein,Protein, Insect,Proteins, Insect