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Essentially complete backbone and side-chain 1H, 15N and 13C resonance assignments for the 185-amino-acid cytokine interleukin-6 (IL-6) are presented. NMR experiments were performed on uniformly [15N]- and [15N,13C]-labeled recombinant human IL-6 (rIL-6) using a variety of heteronuclear NMR experiments. A combination of 13C-chemical shift, amide hydrogen-bond exchange, and 15N-edited NOESY data allowed for analysis of the secondary structure of IL-6. The observed secondary structure of IL-6 is composed of loop regions connecting five alpha-helices, four of which are consistent in their length and disposition with the four-helix bundle motif present in other related cytokines and previously postulated for IL-6. In addition, the topology of the overall fold was found to be consistent with a left-handed up-up-down-down four-helix bundle based on a number of long-range interhelical NOEs. The results presented here provide deeper insight into structure-function relationships among members of the four-helix bundle family of proteins.
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
May 1995,
Biochemistry,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
July 2005,
Journal of biomolecular NMR,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
June 1997,
European journal of biochemistry,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
September 1992,
Biochemistry,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
January 1995,
Biochemistry,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
December 1999,
Journal of biomolecular NMR,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
August 1995,
Journal of magnetic resonance. Series B,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
February 1997,
Protein science : a publication of the Protein Society,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
April 2016,
Biomolecular NMR assignments,
G Y Xu, and
J Hong, and
T McDonagh, and
M Stahl, and
L E Kay, and
J Seehra, and
D A Cumming
March 1994,
Biochemistry,
