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The involvement of phospholipids in the activity of 5alpha-reductase from nuclear and microsomal fractions of rat prostate was studied using phospholipases A and C. Phospholipase A reduced the activity of 5alpha-reductase in both subcellular fractions; this effect was not attributable to the hydrolysis products. Phospholipase C stopped only the activity of the nuclear fraction. There was no quantitative correlation between hydrolysis of phospholipids and loss of enzyme activity. The inactivation observed seems to result from phospholipase-induced instability of the enzyme. The results suggest that the altered structure of phospholipids and/or the membranes where 5alpha-reductase is located is responsible for the decrease in activity.
E H Charreau, and
E M Salmoral
January 1975,
Methods in enzymology,
E H Charreau, and
E M Salmoral
January 1977,
Folia psychiatrica et neurologica japonica,
E H Charreau, and
E M Salmoral
June 1974,
Biochimica et biophysica acta,
E H Charreau, and
E M Salmoral
August 1977,
Biochimica et biophysica acta,
E H Charreau, and
E M Salmoral
March 1978,
Journal of dental research,
E H Charreau, and
E M Salmoral
November 1970,
Acta endocrinologica,
E H Charreau, and
E M Salmoral
January 1977,
Zeitschrift fur Naturforschung. Section C, Biosciences,
E H Charreau, and
E M Salmoral
February 1972,
The Journal of biological chemistry,
E H Charreau, and
E M Salmoral
February 1974,
Steroids,
E H Charreau, and
E M Salmoral
September 1973,
Endocrinology,
