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A novel culture system using biotin/streptavidin and flow cytometry was developed to compare maturation and growth rates in Plasmodium falciparum malaria parasites in two distinct red blood cell (RBC) populations. Biotin was used to label a selected RBC population which was then mixed with another distinct unbiotinylated RBC population. P. falciparum-infected RBCs were used to initiate co-cultures followed over 2-3 schizogonic growth cycles. Co-cultures were harvested and stained with streptavidin-fluorescein isothiocyanate (FITC) followed by fixation and staining of parasite DNA. The combination of biotin/streptavidin-FITC and DNA fluorochrome enabled simultaneous flow cytometric analysis of the two different RBC populations and of the parasitemias in each RBC population. We then used this system to study the in vitro susceptibility of RBCs from individuals with hemoglobin H (Hb H) disease to infection and growth of P. falciparum. Significant reduction in parasite multiplication was found in Hb H RBCs as compared with that in normal RBCs. This novel malaria culture system offers two major innovations: a method to compare directly the relative ability of any two red blood cell populations to support malaria parasite invasion and development under identical conditions, and a critical reduction in the volume of blood and reagents needed to assess parasite growth. The application of biotin-labeled RBCs in the flow cytometric analysis of parasite development may offer new insights in studies of the relationship between RBC defects and susceptibility to malaria parasites.
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
September 1996,
Clinical and laboratory haematology,
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
August 2019,
Transfusion,
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
January 2002,
Methods in cell science : an official journal of the Society for In Vitro Biology,
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
April 1995,
British journal of haematology,
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
February 1994,
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas,
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
September 2022,
Biomedical optics express,
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
February 2008,
Optics communications,
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
July 2019,
Cytometry. Part A : the journal of the International Society for Analytical Cytology,
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
January 2018,
Methods in molecular biology (Clifton, N.J.),
K Pattanapanyasat, and
K Yongvanitchit, and
D G Heppner, and
P Tongtawe, and
D E Kyle, and
H K Webster
February 2000,
Cytometry,
