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/We have studied the effect of interferon alpha (IFN-alpha) on MHC class II expression by human buccal epithelial cells (BEC), and mRNA expression by BEC and mucosal-associated mononuclear cells (MAMC). In 6 experiments, freshly collected BEC were suspended at a concentration of 1.0 x 10(5)/ml in RPMI 1640 and incubated in the presence of 0-10,000 IU/ml of human lymphoblastoid IFN-alpha (HuIFN-alpha). Zero and six hour samples were analyzed by single color flow cytometry using FITC-labeled murine IgG1 monoclonal antibody to HLA-DR. Preparations were also analyzed for expression of cytokine transcripts (IL-2, IL-4, IL-5, IL-6, IL-8, IFN-gamma, GM-CSF) by reverse transcriptase polymerase chain reaction (RT-PCR). Increasing concentrations of IFN-alpha resulted in proportionate increases in the percentage of HLA-DR + BEC (r = 0.7897, p = 0.0627) and in the percentage of HLA-DR+ staining at higher intensities (10(1) to 10(2) log fluorescence intensity) (LFI) (r = 0.4010, p = 0.0424). The percentage of HLA-DR + BEC rose from a mean of 1.5% with no IFN-alpha to 7% with 10,000 IU/ml IFN-alpha (p < 0.05). The percentage of HLA-DR + BEC staining at 10(1) to 10(2) LFI rose from a mean of 8.3% with no added IFN-alpha to 19.2% with 10,000 IU/ml IFN-alpha (p < 0.05). Unstimulated BEC constitutively expressed IL-8 and GM-CSF. IFN-alpha stimulated preparations also expressed IFN-gamma, possibly due to the presence of MAMC, which comprised 2-9% of the total cell population. These data indicated that HuIFN-alpha upregulates MHC class II expression by human BEC, possibly by enhancing IFN-gamma production by MAMC present in the culture preparations.
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
December 1994,
Investigative ophthalmology & visual science,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
October 1986,
The American journal of pathology,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
September 1990,
Pigment cell research,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
June 1986,
Endocrinology,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
January 1991,
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
November 1990,
American journal of respiratory cell and molecular biology,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
May 1988,
The Journal of clinical endocrinology and metabolism,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
July 1989,
Experimental cell research,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
October 1994,
Scandinavian journal of gastroenterology,
J K Smith, and
D S Chi, and
G Krishnaswamy, and
S Srikanth, and
S Reynolds, and
S L Berk
August 1989,
The British journal of ophthalmology,
