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Protein components of a rat brain synaptic junctional protein preparation. 1996
K Langnaese, and
C Seidenbecher, and
H Wex, and
B Seidel, and
K Hartung, and
U Appeltauer, and
A Garner, and
B Voss, and
B Mueller, and
C C Garner, and
E D Gundelfinger
Federal Institute for Neurobiology, Department of Neurochemistry and Molecular Biology, Magdeburg, Germany.
Antisera against a rat brain synaptic protein preparation, the postsynaptic density (PSD) fraction, were used to isolate cDNA clones by expression screening of a rat brain cDNA library. About one fifth of more than 200 analyzed cDNAs encoding potential synapse-associated proteins were previously unknown. Identifiable proteins include, among others, components of the pre- and postsynaptic cytoskeleton, synaptic vesicle proteins and several protein kinases and kinase substrates. This demonstrates that both pre- and postsynaptic elements purify with the PSD fraction.
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