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An analytical method for the determination of cardiac glycosides in Digitalis lanata leaves by reversed-phase thin-layer chromatography (RP-TLC) was developed. The procedure consisted of extraction of dry leaf powder with 50% methanol and clean-up by Sep-Pak cartridges prior to RP-TLC analysis. RP-TLC was performed on an octadecylsilyl bonded silica gel plate, using a developing solvent of acetonitrile-methanol-0.5 M NaCl (1:1:1) for primary glycosides and acetonitrile-methanol-0.5 M NaCl (12:7:9) for secondary glycosides. The plate was scanned with a reflectance densitometer at 225 nm. The quantitation was carried out by the internal standard method. The present method is reliable and relatively simple for the determination of cardiac glycosides in Digitalis lanata leaves.
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
January 1971,
Farmatsevtychnyi zhurnal,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
April 1962,
Svensk farmaceutisk tidskrift,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
November 1965,
Yao xue xue bao = Acta pharmaceutica Sinica,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
January 1963,
Acta poloniae pharmaceutica,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
May 1955,
The Journal of pharmacy and pharmacology,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
July 1986,
Analytical biochemistry,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
October 1983,
Planta medica,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
June 1984,
Planta medica,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
June 1984,
Planta medica,
Y Ikeda, and
Y Fujii, and
M Umemura, and
T Hatakeyama, and
M Morita, and
M Yamazaki
August 1965,
Journal of chromatography,
