Biomaterial Database

Serologically defined lymphocyte alloantigens in Spanish sheep. 1995

J J Garrido, and D F de Andres, and C O Pintado, and D Llanes, and M J Stear

Departmento de Genetica, Facultad de Veterinaria, Universidad de Cordoba, Espana.

Using segregation and population analysis of serological data prepared in our laboratory, we have tentatively defined five OLA antigenic specificities: CO1.1, CO1.2, CO2.1 and CO2.2, whose genetic control would fit a model of two class I OLA loci (CO1.1 and CO1.2 first locus, CO2.1 and CO2.2 second locus, and CO3.1, that could represent a subtype of CO2.2. Alloantisera were obtained from Spanish Merino sheep by intrafamilial cross-immunization and from serum of primiparous and multiparous ewes. Screening of alloantisera for antilymphocyte reactivity was carried out by a two-step dye exclusion microcytotoxicity test against 216 animals belonging to different local breeds: Spanish Merino, Merino Landschaft, Manchega and Churra. Lymphocyte antigen specificities were characterized by cluster and correlation analysis, as well as family studies. Comparison of CO alloantisera with reference reagents from Scotland (CA reagents) showed an excellent correlation between CO1.1 and CA2, and CO2.1 and CA5. The reagents described can be used in various breeds, a characteristic important for their future applications.

UI	MeSH Term	Description	Entries
D007106	Immune Sera	Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.	Antisera,Immune Serums,Sera, Immune,Serums, Immune
D007202	Indicators and Reagents	Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)	Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D007519	Isoantigens	Antigens that exist in alternative (allelic) forms in a single species. When an isoantigen is encountered by species members who lack it, an immune response is induced. Typical isoantigens are the BLOOD GROUP ANTIGENS.	Alloantigens,Alloantigen,Isoantigen
D008297	Male		Males
D012016	Reference Values	The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.	Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D001947	Breeding	The production of offspring by selective mating or HYBRIDIZATION, GENETIC in animals or plants.	Breedings
D005260	Female		Females
D006649	Histocompatibility Antigens	A group of antigens that includes both the major and minor histocompatibility antigens. The former are genetically determined by the major histocompatibility complex. They determine tissue type for transplantation and cause allograft rejections. The latter are systems of allelic alloantigens that can cause weak transplant rejection.	Transplantation Antigens,Antigens, Transplantation,Histocompatibility Antigen,LD Antigens,SD Antigens,Antigen, Histocompatibility,Antigens, Histocompatibility,Antigens, LD,Antigens, SD
D006650	Histocompatibility Testing	Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)	Crossmatching, Tissue,HLA Typing,Tissue Typing,Crossmatchings, Tissue,HLA Typings,Histocompatibility Testings,Testing, Histocompatibility,Testings, Histocompatibility,Tissue Crossmatching,Tissue Crossmatchings,Tissue Typings,Typing, HLA,Typing, Tissue,Typings, HLA,Typings, Tissue
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia