-transparent.png){kind=logo}
An easy, sensitive, competitive indirect enzyme-linked immunosorbent assay (CI-ELISA) for spectinomycin in chicken plasma was developed. Preparation of a spectinomycin-bovine serum albumin conjugate in which the hapten is linked to the carrier protein through the C-4 position is described. Antibodies raised against antigens in rabbits had excellent specificity for spectinomycin, exhibiting a cross-reactivity of 44.0% with dihydrospectinomycin and 13.8% with tetrahydrospectinomycin. No cross-reactivity was observed with other antibiotics. The detection limit of the CI-ELISA was 2 ng/mL (equivalent into 40 ng/mL undiluted chicken plasma) spectinomycin. Known amounts (0.1-100 micrograms/mL) of spectinomycin were added to chicken plasma and then analyzed. Average recoveries were 97-110%. This procedure may be used without prior extraction of samples.
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
February 1991,
Clinical chemistry,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
May 1985,
Journal of steroid biochemistry,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
May 1985,
Journal of steroid biochemistry,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
October 1987,
Journal of lipid research,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
October 1989,
Rinsho byori. The Japanese journal of clinical pathology,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
July 1992,
Chemical & pharmaceutical bulletin,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
January 2001,
Journal of clinical laboratory analysis,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
January 1986,
Folia biologica,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
January 1990,
Methods in enzymology,
T Tanaka, and
H Ikebuchi, and
J I Sawada, and
M Okada, and
Y Kido
January 2000,
Journal of immunoassay,
