-transparent.png){kind=logo}
A broad-host-range promoter-probing vector, pMY3 (8.0 kb), was constructed for cloning of DNA fragments containing promoter sequences of Xanthomonas campestris pv. campestris. This vector (pMY3) consists of the RK2 replicon, promoterless luxAB genes, the thr attenuator to block the transcription of RNA into the luxAB region, and multiple cloning sites for cloning of the fragment carrying promoter sequences. The feasibility of using pMY3 as a promoter-probing vector in both E. coli and Xc17 was demonstrated by using the lac promoter of E. coli, and the amy promoter of X. campestris in Xc17. Among the 63 promoter-containing fragments cloned from Xc17, only 9 were able to express in E. coli. It appears that X. campestris can recognize most E. coli type promoters, but, E. coli can recognize only a small portion of the X. campestris type promoters.
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
February 1990,
Molecular & general genetics : MGG,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
February 1988,
Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
September 2002,
BioTechniques,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
December 1984,
The EMBO journal,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
May 1994,
BioTechniques,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
October 1999,
Wei sheng wu xue bao = Acta microbiologica Sinica,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
October 1990,
Nucleic acids research,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
September 1983,
Gene,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
May 1990,
Gene,
S F Weng, and
M Y Shieh, and
F Y Lai, and
Y Y Shao, and
J W Lin, and
Y H Tseng
January 1983,
Gene,
