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We have isolated a new gene (PTK2) which restores spermine uptake of a polyamine uptake-deficient mutant of Saccharomyces cerevisiae (Kakinuma, Y., Maruyama, T., Nozaki, T., Wada, Y., Oshumi, Y., and Igarashi, K., 1995, Biochem, Biophys. Res. Commun. 216, 985-992). In magnesium-limited medium, the cell growth of a spermine-sensitive polyamine uptake mutant transformed with PTK2 recovered its sensitivity to spermine. The nucleotide sequence of the PTK2 gene indicated that it is identical with the YJR059W open reading frame of chromosome X encoding a putative serine/threonine protein kinase. The deduced amino acid sequence of the PTK2 gene product was 38% identical and 55% similar with that of the PTK1 (POT1) gene product, a putative serine/threonine protein kinase, which was found to enhance spermine uptake of the same mutant. The results indicate that polyamine transport of yeast is regulated by multiple phosphorylation/dephosphorylation pathways.
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
November 1995,
Biochemical and biophysical research communications,
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
January 1992,
Yeast (Chichester, England),
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
May 1992,
Gene,
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
May 1993,
Yeast (Chichester, England),
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
June 1997,
Journal of bacteriology,
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
December 1993,
Current biology : CB,
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
March 1997,
Molecular and biochemical parasitology,
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
November 2007,
Genetica,
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
May 1997,
FEBS letters,
T Nozaki, and
K Nishimura, and
A J Michael, and
T Maruyama, and
Y Kakinuma, and
K Igarashi
May 1994,
Biochimica et biophysica acta,
