Biomaterial Database

A procedure for isolation of pure complexes of acylated and unacylated tRNA(Phe) with ribosomes from Escherichia coli. 1996

G Chinali

Dipartimento di Medicina Clinica e Sperimentale, Facoltà di Medicina e Chirurgia, Università di Napoli Federico II, Napoles, Italy.

A simple and rapid procedure for the isolation of pure specific complexes of poly(U).ribosome with acylated and/or unacylated tRNA(Phe) is described. The method is based on the use of conditions that favor the formation of polyribosomes. The polyribosomes are separated from ribosomal subunits and monoribosomes by gel filtration on Sepharose 4B at low Mg2+ concentrations that cause the selective dissociation of tRNA-vacant ribosomes from polyribosomes. The procedure allows the isolation in about one hour of various ribosome.poly(U).tRNA complexes completely free of ribosomal particles unable to bind tRNA. A minor fraction of the purified ribosomal complexes is formed by particles devoid of peptidyltransferase activity. Attempts to eliminate this fraction of inactive ribosomes and to obtain ribosomal complexes fully active in polypeptide synthesis were unsuccessful.

UI	MeSH Term	Description	Entries
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000215	Acylation	The addition of an organic acid radical into a molecule.
D012270	Ribosomes	Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.	Ribosome
D012329	RNA, Bacterial	Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.	Bacterial RNA
D012360	RNA, Transfer, Phe	A transfer RNA which is specific for carrying phenylalanine to sites on the ribosomes in preparation for protein synthesis.	Phenylalanine-Specific tRNA,Transfer RNA, Phe,tRNAPhe,tRNA(Phe),Phe Transfer RNA,Phenylalanine Specific tRNA,RNA, Phe Transfer,tRNA, Phenylalanine-Specific