BIOMAT Database Logo BIOMAT Database Logo

Axonal origin and purity of growth cones isolated from fetal rat brain. 1996

K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Department of Cellular and Structural Biology, University of Colorado School of Medicine, Denver 80262, USA.

The investigation of the molecular properties of nerve growth cones depends to a significant degree on their isolation from fetal brain in the form of 'growth cone particles' (GCPs). The availability of markers for developing axons and dendrites, as well as glial cells, has made it possible to characterize the GCP fraction in much greater detail than before and to optimize its yield. Marker analyses show that a member of the N-CAM family (5B4-CAM), synaptophysin, and especially GAP-43 and non-phosphorylated tau, are enriched in the GCP fraction. In contrast, MAP2 and, particularly, glial fibrillary acidic protein and vimentin are fractionated away from GCPs. Furthermore, GCP yield can be doubled relative to the original procedure, without compromising purity, by raising the sucrose concentration of the fractionation gradient's uppermost layer. The results indicate that GCPs are highly purified growth cone fragments with very little glial contamination, and that they are primarily of axonal origin.

UI MeSH Term Description Entries
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008869 Microtubule-Associated Proteins High molecular weight proteins found in the MICROTUBULES of the cytoskeletal system. Under certain conditions they are required for TUBULIN assembly into the microtubules and stabilize the assembled microtubules. Ensconsin,Epithelial MAP, 115 kDa,Epithelial Microtubule-Associate Protein, 115 kDa,MAP4,Microtubule Associated Protein,Microtubule Associated Protein 4,Microtubule Associated Protein 7,Microtubule-Associated Protein,Microtubule-Associated Protein 7,E-MAP-115,MAP1 Microtubule-Associated Protein,MAP2 Microtubule-Associated Protein,MAP3 Microtubule-Associated Protein,Microtubule Associated Proteins,Microtubule-Associated Protein 1,Microtubule-Associated Protein 2,Microtubule-Associated Protein 3,7, Microtubule-Associated Protein,Associated Protein, Microtubule,E MAP 115,Epithelial Microtubule Associate Protein, 115 kDa,MAP1 Microtubule Associated Protein,MAP2 Microtubule Associated Protein,MAP3 Microtubule Associated Protein,Microtubule Associated Protein 1,Microtubule Associated Protein 2,Microtubule Associated Protein 3,Microtubule-Associated Protein, MAP1,Microtubule-Associated Protein, MAP2,Microtubule-Associated Protein, MAP3,Protein 7, Microtubule-Associated,Protein, Microtubule Associated,Protein, Microtubule-Associated
D009419 Nerve Tissue Proteins Proteins, Nerve Tissue,Tissue Proteins, Nerve
D009457 Neuroglia The non-neuronal cells of the nervous system. They not only provide physical support, but also respond to injury, regulate the ionic and chemical composition of the extracellular milieu, participate in the BLOOD-BRAIN BARRIER and BLOOD-RETINAL BARRIER, form the myelin insulation of nervous pathways, guide neuronal migration during development, and exchange metabolites with neurons. Neuroglia have high-affinity transmitter uptake systems, voltage-dependent and transmitter-gated ion channels, and can release transmitters, but their role in signaling (as in many other functions) is unclear. Bergmann Glia,Bergmann Glia Cells,Bergmann Glial Cells,Glia,Glia Cells,Satellite Glia,Satellite Glia Cells,Satellite Glial Cells,Glial Cells,Neuroglial Cells,Bergmann Glia Cell,Bergmann Glial Cell,Cell, Bergmann Glia,Cell, Bergmann Glial,Cell, Glia,Cell, Glial,Cell, Neuroglial,Cell, Satellite Glia,Cell, Satellite Glial,Glia Cell,Glia Cell, Bergmann,Glia Cell, Satellite,Glia, Bergmann,Glia, Satellite,Glial Cell,Glial Cell, Bergmann,Glial Cell, Satellite,Glias,Neuroglial Cell,Neuroglias,Satellite Glia Cell,Satellite Glial Cell,Satellite Glias
D009836 Oligodendroglia A class of large neuroglial (macroglial) cells in the central nervous system. Oligodendroglia may be called interfascicular, perivascular, or perineuronal (not the same as SATELLITE CELLS, PERINEURONAL of GANGLIA) according to their location. They form the insulating MYELIN SHEATH of axons in the central nervous system. Interfascicular Oligodendroglia,Oligodendrocytes,Perineuronal Oligodendroglia,Perineuronal Satellite Oligodendroglia Cells,Perivascular Oligodendroglia,Satellite Cells, Perineuronal, Oligodendroglia,Perineuronal Satellite Oligodendrocytes,Interfascicular Oligodendroglias,Oligodendrocyte,Oligodendrocyte, Perineuronal Satellite,Oligodendrocytes, Perineuronal Satellite,Oligodendroglia, Interfascicular,Oligodendroglia, Perineuronal,Oligodendroglia, Perivascular,Perineuronal Satellite Oligodendrocyte,Satellite Oligodendrocyte, Perineuronal,Satellite Oligodendrocytes, Perineuronal
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002458 Cell Fractionation Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS. Cell Fractionations,Fractionation, Cell,Fractionations, Cell
D005314 Embryonic and Fetal Development Morphological and physiological development of EMBRYOS or FETUSES. Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal
D005904 Glial Fibrillary Acidic Protein An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000. Glial Intermediate Filament Protein,Astroprotein,GFA-Protein,Glial Fibrillary Acid Protein,GFA Protein

Related Publications

K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Regulated release of serotonin from axonal growth cones isolated from the fetal rat brain.
January 1998, Neurochemistry international,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Nerve growth cones isolated from fetal rat brain: subcellular fractionation and characterization.
December 1983, Cell,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Nerve growth cones isolated from fetal rat brain. III. Calcium-dependent protein phosphorylation.
June 1985, The Journal of neuroscience : the official journal of the Society for Neuroscience,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
A chloride channel reconstituted from fetal rat brain growth cones.
July 1994, The Journal of membrane biology,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Biochemical characterization of nerve growth cones isolated from both fetal and neonatal rat forebrains: the growth cone particle fraction mainly consists of axonal growth cones in both stages.
February 1992, Brain research. Developmental brain research,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Activity of sialidases in fetal brain axonal growth cones and during postnatal development.
February 1999, International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Phosphoprotein B-50 in nerve growth cones from fetal rat brain.
November 1985, Neuroscience letters,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Biochemical properties of Na+/K(+)-ATPase in axonal growth cone particles isolated from fetal rat brain.
August 1994, International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
Uptake and release of [3H]GABA by growth cones isolated from neonatal rat brain.
November 1984, Neuroscience letters,
K Lohse, and S M Helmke, and M R Wood, and S Quiroga, and B A de la Houssaye, and V E Miller, and P Negre-Aminou, and K H Pfenninger
GABA release from mouse axonal growth cones.
March 2000, The Journal of physiology,
Copied contents to your clipboard!