Three groups of extracted human teeth were sectioned longitudinally into experimental and control halves. The enamel surfaces of the three groups of teeth were pretreated with 37% H3PO4 for 1 min, 0.5 M NaClO3 for 2 h, or water rinsing for 10 min. The experimental halves of the three groups received an application of 1% TiF4 for 1 min and were washed with deionized water. The tooth specimens were then immersed in synthetic saliva for 24 h at 37 degrees C before sampling for measurement of fluoride uptake. An acid etching technique was used to determine the uptake of fluoride by measuring both fluoride and calcium concentrations in the solution collected from each etched sample. A significantly higher uptake of fluoride was found in teeth rinsed in water and etched with 37% H3PO4 than in teeth pretreated in 0.5 M NaClO3. It seems that the organic components in enamel play an important role in the fluoride uptake after the topical application of TiF4.