-transparent.png){kind=logo}
Immunoaffinity extraction for the high-performance liquid chromatographic determination of equilin and its metabolites in plasma has been achieved. The antibody raised against an equilin 3-O-carboxymethyl ether-bovine serum albumin conjugate was characterized as having a high affinity for equilin and equilenin. One mL of the immunoaffinity adsorbent prepared by immobilization of an antibody was capable of retaining up to 1 microgram of equilin and equilenin, to 100 ng of other metabolites including 2-methoxylated and 17 beta-reduced compounds, and to 0.3 micrograms of glucuronic acid conjugates at C-3. The adsorbates were recovered qualitatively by elution with 90% aqueous (v/v) methanol without any interfering peaks on the chromatogram.
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
March 1994,
Journal of pharmaceutical and biomedical analysis,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
May 1984,
Clinical chemistry,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
April 1996,
Journal of chromatography. B, Biomedical applications,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
January 2003,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
January 1984,
Arzneimittel-Forschung,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
January 1989,
Journal of pharmaceutical and biomedical analysis,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
March 1998,
Journal of chromatography. B, Biomedical sciences and applications,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
May 1984,
Clinical chemistry,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
January 1982,
Pharmacology,
S Ikegawa, and
M Itoh, and
N Murao, and
H Kijima, and
M Suzuki, and
T Fujiyama, and
J Goto
June 1995,
Journal of chromatography. B, Biomedical applications,
