Biomaterial Database

[Cytomegalovirus infection in patients after kidney transplantation]. 1996

M Nouza, and L Korcáková

Klinika nefrologie IKEM, Praha.

BACKGROUND Cytomegalovirus disease (CMV) (active infection with various clinical and laboratory symptoms) in patients after renal transplantation (Tx) is an important diagnostic and therapeutic problem. Early CMV antigen pp65 is a structural protein of the antigenic type. It was discovered as a marker of activation of CMV infection. We decided to test the value of the method of early CMV antigen detection in the diagnosis of CMV disease in patients after renal transplantation (Tx). RESULTS During a 10-month period 53 patients after Tx of the kidney were examined for the presence of early CMV antigen. Thirty-nine patients were followed up systematically, examined for the first time before Tx and then after regular one-week intervals. All patients were subjected to serological examination before Tx for the presence of antibodies against CMV, class IgG; in 41 during subsequent examinations also IgM antibodies were assessed. A method for the assessment of early CMV antigen was elaborated by cultivation of biological material in a tissue culture using monoclonal antibodies and indirect immunofluorescence. In the mentioned 53 patients a total of 363 examinations were made, mainly in blood and urine, 63 samples were positive Among the 39 patients followed up systematically early antigen was positive in 17 patients (44%). In three already before Tx. Positivity of the early antigen was first revealed on average 16 days (+/-4.2) after Tx and persisted on average for 13 days (+/-6.4). Positive early antigen was associated in the majority of patients with one or several clinical and laboratory symptoms, most frequently elevated body temperature. Comparison of early antigen and IgM anti-CMV were statistically evaluated by the chi sq. test, Fisher's test in contingency tables and by Student's non-parametric t-test. CONCLUSIONS We assembled new experience with the method of detection of early CMV antigen in the diagnosis of activation of CMV infection. The correlation of the method with clinical and laboratory symptoms, incl. anti-CMV IGM antibodies, was statistically significant. By this method it is possible to detect CMV activation on average already on the 16th day after Tx which contributes in a significant way to the early diagnosis of the disease and facilitates the differential diagnosis of conditions during the early postoperative period.

UI	MeSH Term	Description	Entries
D009894	Opportunistic Infections	An infection caused by an organism which becomes pathogenic under certain conditions, e.g., during immunosuppression.	Infection, Opportunistic,Infections, Opportunistic,Opportunistic Infection
D003586	Cytomegalovirus Infections	Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults.	CMV Inclusion,CMV Inclusions,Congenital CMV Infection,Congenital Cytomegalovirus Infection,Cytomegalic Inclusion Disease,Cytomegalovirus Colitis,Cytomegalovirus Inclusion,Cytomegalovirus Inclusion Disease,Cytomegalovirus Inclusions,Inclusion Disease,Perinatal CMV Infection,Perinatal Cytomegalovirus Infection,Renal Tubular Cytomegalovirus Inclusion,Renal Tubular Cytomegalovirus Inclusions,Salivary Gland Virus Disease,Severe Cytomegalovirus Infection,Severe Cytomegalovirus Infections,Infections, Cytomegalovirus,CMV Infection, Congenital,CMV Infection, Perinatal,Colitis, Cytomegalovirus,Congenital CMV Infections,Congenital Cytomegalovirus Infections,Cytomegalic Inclusion Diseases,Cytomegalovirus Colitides,Cytomegalovirus Inclusion Diseases,Cytomegalovirus Infection,Cytomegalovirus Infection, Congenital,Cytomegalovirus Infection, Perinatal,Cytomegalovirus Infection, Severe,Cytomegalovirus Infections, Severe,Disease, Cytomegalic Inclusion,Disease, Cytomegalovirus Inclusion,Diseases, Cytomegalovirus Inclusion,Inclusion Disease, Cytomegalic,Inclusion Disease, Cytomegalovirus,Inclusion Diseases,Inclusion Diseases, Cytomegalovirus,Inclusion, CMV,Inclusion, Cytomegalovirus,Infection, Congenital CMV,Infection, Congenital Cytomegalovirus,Infection, Cytomegalovirus,Infection, Perinatal CMV,Infection, Perinatal Cytomegalovirus,Infection, Severe Cytomegalovirus,Perinatal CMV Infections,Perinatal Cytomegalovirus Infections
D003587	Cytomegalovirus	A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.	Herpesvirus 5, Human,Human Herpesvirus 5,Salivary Gland Viruses,HHV 5,Herpesvirus 5 (beta), Human,Cytomegaloviruses,Salivary Gland Virus,Virus, Salivary Gland,Viruses, Salivary Gland
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000956	Antigens, Viral	Substances elaborated by viruses that have antigenic activity.	Viral Antigen,Viral Antigens,Antigen, Viral
D014776	Virus Cultivation	Process of growing viruses in live animals, plants, or cultured cells.	Viral Cultivation,Cultivation, Viral,Cultivation, Virus,Cultivations, Viral,Cultivations, Virus,Viral Cultivations,Virus Cultivations
D016030	Kidney Transplantation	The transference of a kidney from one human or animal to another.	Grafting, Kidney,Renal Transplantation,Transplantation, Kidney,Transplantation, Renal,Kidney Grafting,Kidney Transplantations,Renal Transplantations,Transplantations, Kidney,Transplantations, Renal
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016867	Immunocompromised Host	A human or animal whose immunologic mechanism is deficient because of an immunodeficiency disorder or other disease or as the result of the administration of immunosuppressive drugs or radiation.	Immunosuppressed Host,Immunocompromised Patient,Host, Immunocompromised,Host, Immunosuppressed,Hosts, Immunocompromised,Hosts, Immunosuppressed,Immunocompromised Hosts,Immunocompromised Patients,Immunosuppressed Hosts,Patient, Immunocompromised,Patients, Immunocompromised
D017403	In Situ Hybridization	A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.	Hybridization in Situ,Hybridization, In Situ,Hybridizations, In Situ,In Situ Hybridizations