Evidence for receptor-mediated mineralocorticoid action in rat osteoblastic cells. 1996

M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
Hormone Laboratory, Institut National de la Santé et de la Recherche Médicale Unité 86, Centre Universitaire des Cordeliers, Paris, France.

Aldosterone significantly enhanced the proliferation of osteoblastic cells from rat calvaria, and this effect was inhibited by RU 26752 and ZK 91587, two antagonists specific to the mineralocorticoid receptor (MCR). In addition, aldosterone inhibited the activity of alkaline phosphatase, a marker of the osteoblastic phenotype, and this effect was also reversed by RU 26752. Cytoplasmic staining of MCR was observed in rat calvaria osteoblasts incubated with a specific polyclonal antiserum raised against rat kidney MCR. This anti-MCR immunoglobulin G immunoprecipitated and macroaggregated the MCR-[3H]RU 26752 complex in osteoblastic cytosol. A single 98-kDa band was observed when osteoblastic cytosol was analyzed by Western blotting with anti-MCR serum. The 98-kDa band was also obtained after autoradiography of irradiated osteoblastic cytosol-[3H]R 5020 complex, and this was abolished in the presence of RU 26752. A p26MR probe, specific to COOH-terminal end of MCR, hybridized with the predicted product after amplification of total cell RNA by polymerase chain reaction technique. Furthermore, hybridization of poly(A)+ mRNA from at calvaria osteoblastic cells with the p26MR probe revealed a major band of approximately 4.2 kb. Collectively, our studies demonstrate the existence of a functional MCR in rat calvaria osteoblasts.

UI MeSH Term Description Entries
D008901 Mineralocorticoids A group of CORTICOSTEROIDS primarily associated with water and electrolyte balance. This is accomplished through the effect on ION TRANSPORT in renal tubules, resulting in retention of sodium and loss of potassium. Mineralocorticoid secretion is itself regulated by PLASMA VOLUME, serum potassium, and ANGIOTENSIN II. Mineralocorticoid,Mineralocorticoid Effect,Mineralocorticoid Effects,Effect, Mineralocorticoid,Effects, Mineralocorticoid
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010006 Osteoblasts Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone. Osteoblast
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D000450 Aldosterone A hormone secreted by the ADRENAL CORTEX that regulates electrolyte and water balance by increasing the renal retention of sodium and the excretion of potassium. Aldosterone, (+-)-Isomer,Aldosterone, (11 beta,17 alpha)-Isomer
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D013256 Steroids A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed) Steroid,Catatoxic Steroids,Steroids, Catatoxic

Related Publications

M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
March 1994, Pharmacological reviews,
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
January 1980, Journal of steroid biochemistry,
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
October 1988, Science (New York, N.Y.),
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
November 2005, Molecular and cellular endocrinology,
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
February 1992, The Physiologist,
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
November 2005, Biochemical and biophysical research communications,
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
February 2016, Biochimica et biophysica acta,
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
April 1988, European journal of pharmacology,
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
February 1993, The Physiologist,
M K Agarwal, and F Mirshahi, and M Mirshahi, and S Bracq, and J Chentoufi, and M Hott, and A Jullienne, and P J Marie
September 1992, Circulation research,
Copied contents to your clipboard!