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Two galactose-binding lectins, SL1 and SL2, were isolated from human serum by two-step affinity chromatography on sorbents with immobilized disaccharides Gal beta 1-3GlcNAc beta (Lec) and Fuc alpha 1-2Gal beta (Hdi). The purification degree of the SL1 and SL2 preparations was 4000-6000 times and yields were 6.9 and 4.7 micrograms/ml of serum, respectively. Electrophoresis showed that both lectins are oligomers with molecular masses of about 440 kDa, which are composed of ca. 67 kDa subunits. The carbohydrate specificity of the lectins was assayed by hemagglutination inhibition using mono- and oligosaccharides. Both lectins showed specificity for Gal and GalNAc residues but differed in the interactions with oligosaccharides. In particular, SL1 showed maximum affinity for disaccharide Gal beta 1-3GalNAc beta (in the form of 4-nitrophenyl glycoside), 6'-sialyllactose, and disaccharide residues Fuc alpha 1-3Gal beta and Gal alpha 1-3Gal beta, whereas SL2 was specific for GalNAc alpha residue and its derivatives as well as for disaccharide Hdi.
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
February 1974,
The Journal of biological chemistry,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
June 2014,
Acta crystallographica. Section F, Structural biology communications,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
October 1994,
European journal of cell biology,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
March 1977,
Biochemical and biophysical research communications,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
September 1987,
Biochimica et biophysica acta,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
November 1980,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
October 1992,
Comparative biochemistry and physiology. B, Comparative biochemistry,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
January 2015,
Current protein & peptide science,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
January 1993,
Histochemistry,
E M Rapoport, and
L S Zhigis, and
E Iu Korchagina, and
T V Ovchinnikova, and
V P Zubov, and
N V Bovin
January 2003,
Zhurnal evoliutsionnoi biokhimii i fiziologii,
