Biomaterial Database

Glycoproteins with N-acetylglucosamine and mannose residues in Chinese hamster metaphase chromosomes. 1996

J Myllyharju, and S Nokkala

Department of Biology, University of Turku, Finland.

Distribution of glycosylated proteins in Chinese hamster metaphase chromosomes was studied with fluorescein isothiocyanate conjugated lectins. Three substructural domains with distinct glycoprotein compositions were identified. In situ binding of the lectins Wheat germ agglutinin (WGA) and Datura stramonium agglutinin (DSA) showed that chromosomal proteins containing N-acetylglucosamine residues preferentially localize to the surface domain and the helically coiled substructure of chromatids. In Western blots, digoxigenin conjugated WGA and DSA bound to several chromosomal proteins with molecular weight ranges of 45-220 kD and 66-220 kD, respectively. Binding of Galanthus nivalis agglutinin revealed that mannosylated chromosomal proteins are enriched at the surface and G/Q band domains, and their molecular weights range from 97 to 200 kD. The carbohydrate side chain structure of these mannosylated proteins must be quite specific, as after binding of another mannose-specific lectin, Concanavalin A, only a faint fluorescence was observed in metaphase chromosomes and only one protein band of 185 kD was weakly stained in Western blots. These data suggest that chromosomal glycoproteins containing N-acetylglucosamine and mannose residues play a role in the higher order structural organization of metaphase chromosomes.

UI	MeSH Term	Description	Entries
D008358	Mannose	A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)	D-Mannose,Mannopyranose,Mannopyranoside,D Mannose
D008677	Metaphase	The phase of cell nucleus division following PROMETAPHASE, in which the CHROMOSOMES line up across the equatorial plane of the SPINDLE APPARATUS prior to separation.
D002875	Chromosomes	In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)	Chromosome
D003412	Cricetulus	A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.	Hamsters, Armenian,Hamsters, Chinese,Hamsters, Grey,Armenian Hamster,Armenian Hamsters,Chinese Hamster,Chinese Hamsters,Grey Hamster,Grey Hamsters,Hamster, Armenian,Hamster, Chinese,Hamster, Grey
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster
D000117	Acetylglucosamine	The N-acetyl derivative of glucosamine.	Acetyl Glucosamine,N-Acetyl Glucosamine,N-Acetyl-beta-D-Glucosamine,N-Acetylglucosamine,beta-N-Acetylglucosamine,2-Acetamido-2-Deoxy-D-Glucose,2-Acetamido-2-Deoxyglucose,N-Acetyl-D-Glucosamine,2 Acetamido 2 Deoxy D Glucose,2 Acetamido 2 Deoxyglucose,Glucosamine, Acetyl,Glucosamine, N-Acetyl,N Acetyl D Glucosamine,N Acetyl Glucosamine,N Acetyl beta D Glucosamine,N Acetylglucosamine,beta N Acetylglucosamine
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015153	Blotting, Western	Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.	Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D027801	Galanthus	A plant genus in the family Amaryllidaceae. Galanthus nivalis L. is the source of GALANTHAMINE.	Galanthus nivalis,Snowdrops,Snowdrop