Biomaterial Database

High-sensitivity device for continuous measurements of tissue O2 uptake. 1977

R Biron, and A Dittmar

An analysis system for continuous measurements of O2 uptake by rat diaphragm during several hours is described. The tissue was set in a chamber where a medium was flowing at a known and constant flow rate, temperature, and partial pressure of oxygen (PO2). The decrease in PO2 of the medium was measured with a Clark O2 cathode at the outlet of the chamber. To increase the sensitivity of the system and the signal-to-noise ratio of the measurements, a sweeping device was designed. It provided homogenization of the medium in the chamber. The main characteristics of this analysis system are a) a sensitivity of +/-0.2% of the mean PO2 value of the perfused medium, +/-0.8% of the mean O2 uptake of rat diaphragm, b) a linearity of +/-1.75% in the range of 0+/-200 Torr, c) a stability of +/-0.38% of the initial PO2 value, and d) a time constant of 8 min 30 s. This type of system can be used for high-sensitivity measurements of O2 uptake of any tissue after adaptation of the chamber and of the medium flow rate.

UI	MeSH Term	Description	Entries
D010101	Oxygen Consumption	The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346)	Consumption, Oxygen,Consumptions, Oxygen,Oxygen Consumptions
D011048	Polarography	An electrochemical technique for measuring the current that flows in solution as a function of an applied voltage. The observed polarographic wave, resulting from the electrochemical response, depends on the way voltage is applied (linear sweep or differential pulse) and the type of electrode used. Usually a mercury drop electrode is used.	DC Polarography,Pulse Polarography,Polarography, DC,Polarography, Pulse
D003964	Diaphragm	The musculofibrous partition that separates the THORACIC CAVITY from the ABDOMINAL CAVITY. Contraction of the diaphragm increases the volume of the thoracic cavity aiding INHALATION.	Respiratory Diaphragm,Diaphragm, Respiratory,Diaphragms,Diaphragms, Respiratory,Respiratory Diaphragms
D004780	Environment, Controlled	A state in which the environs of hospitals, laboratories, domestic and animal housing, work places, spacecraft, and other surroundings are under technological control with regard to air conditioning, heating, lighting, humidity, ventilation, and other ambient features. The concept includes control of atmospheric composition. (From Jane's Aerospace Dictionary, 3d ed)	Clean Rooms,Laminar Air-Flow Areas,Controlled Environment,Area, Laminar Air-Flow,Clean Room,Controlled Environments,Environments, Controlled,Laminar Air Flow Areas,Laminar Air-Flow Area,Room, Clean
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus