BIOMAT Database Logo BIOMAT Database Logo

High pressure liquid chromatographic determination of ethopabate in feeds. 1977

L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway

A high pressure liquid chromatographic (HPLC) method is described for determining ethopabate in poultry feeds. Feed samples containing ethopabate are finely ground, extracted 30 min by sonification with methanol-water (80 + 20), and filtered. The extract is cleaned up on an alumina column, and the first 4 ml eluate collected is analyzed. The drug is eluted through a muBondapak C18 column with acetonitrile-water (30 + 70) at a flow rate of 1.4 ml/min and detected by ultraviolet absorption at 280 nm. Chromatography is complete in 7 min, and detector response is linear. The drug is quantitated by peak height ratios. The procedure described is reproducible and shows good agreement with the official AOAC colorimetric method. The lower limit of detection is 2 ng by HPLC, making the method applicable to residue analyses.

UI MeSH Term Description Entries
D011200 Poultry Domesticated birds raised for food. It typically includes CHICKENS; TURKEYS, DUCKS; GEESE; and others. Fowls, Domestic,Domestic Fowl,Domestic Fowls,Fowl, Domestic,Poultries
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005011 Ethopabate An inhibitor of folate metabolism. It is used as a coccidiostat in poultry.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000821 Animal Feed Foodstuff used especially for domestic and laboratory animals, or livestock. Fodder,Animal Feeds,Feed, Animal,Feeds, Animal,Fodders
D062365 Aminobenzoates Derivatives of BENZOIC ACID that contain one or more amino groups attached to the benzene ring structure. Included under this heading are a broad variety of acid forms, salts, esters, and amides that include the aminobenzoate structure. Aminobenzoic Acids,Acids, Aminobenzoic

Related Publications

L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
High pressure liquid chromatographic determination of nifursol in finished feeds.
July 1981, Journal - Association of Official Analytical Chemists,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
High pressure liquid chromatographic determination of bacitracin in premix feeds and finished feeds: collaborative study.
September 1982, Journal - Association of Official Analytical Chemists,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
High pressure liquid chromatographic determination of xanthomegnin in grains and animal feeds.
May 1983, Journal - Association of Official Analytical Chemists,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
The determination of ethopabate in feeds.
July 1967, The Analyst,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
Gas-liquid chromatographic determination of ethopabate in feed premixes.
May 1978, Journal - Association of Official Analytical Chemists,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
High-performance liquid chromatographic determination of Olaquindox in feeds.
April 1979, Journal of chromatography,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
High pressure liquid chromatographic determination of sulfanitran and dinsed in medicated feeds and premixes.
September 1977, Journal - Association of Official Analytical Chemists,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
Sample preparation of carbadox, furazolidone, nitrofurazone, and ethopabate in medicated feeds for high pressure liquid chromatography.
September 1980, Journal - Association of Official Analytical Chemists,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
High pressure liquid chromatographic determination and fluorescence detection of aflatoxins in corn and dairy feeds.
November 1981, Journal - Association of Official Analytical Chemists,
L R Schronk, and B M Colvin, and A R Hanks, and R J Bushway
Liquid chromatographic determination of sulfamethazine in feeds.
January 1988, Journal - Association of Official Analytical Chemists,
Copied contents to your clipboard!