Biomaterial Database

Hypoxic effects on cholesterol metabolism of cultured rat aortic and brain microvascular endothelial cells, and aortic vascular smooth muscle cells. 1996

Y Arai, and M Sasaki, and N Sakuragawa

Department of Inherited Metabolic Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.

We investigated hypoxic effects on cholesterol metabolism in cultured brain microvascular endothelial cells (BEC), aortic endothelial cells (AEC) and aortic vascular smooth muscle cells (VSMC) from rat. In control conditions, acid lipase activities in BEC and AEC were higher than that in VSMC. Acyl-Coenzyme A: cholesterol acyltransferase (ACAT) activities in control cells of BEC and AEC were lower than that of VSMC. Hypoxic treatment caused decreased acid lipase activity. ACAT activity decreased in VSMC. High pressure lipid chromatography (HPLC) study showed that hypoxia caused decreased contents of cholesterol and cholesteryl esters especially in AEC. We suggested that there is different cholesterol metabolism in the hypoxic treatment among endothelial cells and smooth muscle cells.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D008833	Microcirculation	The circulation of the BLOOD through the MICROVASCULAR NETWORK.	Microvascular Blood Flow,Microvascular Circulation,Blood Flow, Microvascular,Circulation, Microvascular,Flow, Microvascular Blood,Microvascular Blood Flows,Microvascular Circulations
D009131	Muscle, Smooth, Vascular	The nonstriated involuntary muscle tissue of blood vessels.	Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002560	Cerebrovascular Circulation	The circulation of blood through the BLOOD VESSELS of the BRAIN.	Brain Blood Flow,Regional Cerebral Blood Flow,Cerebral Blood Flow,Cerebral Circulation,Cerebral Perfusion Pressure,Circulation, Cerebrovascular,Blood Flow, Brain,Blood Flow, Cerebral,Brain Blood Flows,Cerebral Blood Flows,Cerebral Circulations,Cerebral Perfusion Pressures,Circulation, Cerebral,Flow, Brain Blood,Flow, Cerebral Blood,Perfusion Pressure, Cerebral,Pressure, Cerebral Perfusion
D002784	Cholesterol	The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.	Epicholesterol
D004730	Endothelium, Vascular	Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.	Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000860	Hypoxia	Sub-optimal OXYGEN levels in the ambient air of living organisms.	Anoxia,Oxygen Deficiency,Anoxemia,Deficiency, Oxygen,Hypoxemia,Deficiencies, Oxygen,Oxygen Deficiencies
D001011	Aorta	The main trunk of the systemic arteries.	Aortas