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Mutant alphabeta TCRs were generated by replacing domains of the alpha and beta chain constant regions with homologous domains from TCR delta and gamma chains, respectively. Chimeric TCRs in which the alpha chain contains TCR delta chain sequences within the connecting peptide domain are unresponsive to alloantigens and superantigens, and have defective interactions with the CD3/zeta complex. Although these antigen-unresponsive TCRs undergo zeta chain phosphorylation upon stimulation with superantigen, they do not generate a full signal capable of producing IL-2. Mutant TCRs acquire signaling activity with a combination of superantigen and calcium ionophore, indicating a defect in calcium-mediated signaling. Finally, a conserved motif, FETDxNLN, present in the alpha chain connecting peptide domain, is disrupted in all signaling-defective TCRs. This conserved alpha chain connecting peptide motif might mediate the transfer of signals from the alphabeta heterodimer to the CD3/zeta complex.
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
February 2001,
The Journal of biological chemistry,
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
December 2014,
Monoclonal antibodies in immunodiagnosis and immunotherapy,
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
June 2009,
Immunity,
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
June 2001,
European journal of immunology,
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
January 1984,
Nature,
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
June 2008,
Journal of immunology (Baltimore, Md. : 1950),
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
September 2002,
Journal of immunology (Baltimore, Md. : 1950),
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
July 2010,
Journal of molecular biology,
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
August 1996,
Immunology,
B T Bäckström, and
E Milia, and
A Peter, and
B Jaureguiberry, and
C T Baldari, and
E Palmer
January 1997,
Immunogenetics,
