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Various amplitude normalization procedures for surface electromyographic (EMG) signals have been utilized to reduce the inter- and intrasubject variability of data collected on gait analysis. We examined two normalization methods (normalization to mean of ensemble average: NME, and normalization to peak of ensemble average: NPE) for intersubject variability and reproducibility surface EMG profiles for the masticatory muscles during unilateral gum chewing. The EMG profiles for the anterior temporal (Ta), posterior temporal (Tp), masseter (M) and anterior belly of the digastric (Da) muscles on the chewing side from five normal subjects were made at three different sessions. Coefficients of variation and intraclass correlation coefficients were used to assess variability and reproducibility. Each normalization procedure the intersubject variability of unnormalized EMG profiles for Ta, Tp and M. The NME procedure provided lower intersubject variability for Da. The reproducibility of unnormalized profiles for Ta, Tp and M was significantly improved by both procedures (p < 0.05). NME on Da was more effective in its reproducibility (p < 0.05). We found that the phasic characteristics of muscular activity influenced the outcome of amplitude normalization.
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