Biomaterial Database

Genetic evidence for the relationship of Porphyromonas gingivalis cysteine protease and hemagglutinin activities. 1996

M Yoneda, and H K Kuramitsu

Department of Oral Biology, State University of New York, Buffalo, USA.

Cysteine protease and hemagglutinin activities of Porphyromonas gingivalis have been implicated as virulence factors in periodontitis. In addition, a close structural relationship between these factors has been suggested. In order to examine the molecular basis for such a relationship, we constructed an isogenic mutant, G-102, of P. gingivalis 381 deficient in Arg-gingipain cysteine protease activity. The mutant displayed not only reduced protease activity but also significantly reduced hemagglutination activity compared with the wild-type strain. Therefore, this investigation provided genetic evidence for the recently proposed structural relationship between Arg-gingipain and the hemagglutinin activity of P. gingivalis strains.

UI	MeSH Term	Description	Entries
D010957	Plasmids	Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.	Episomes,Episome,Plasmid
D011995	Recombination, Genetic	Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.	Genetic Recombination,Recombination,Genetic Recombinations,Recombinations,Recombinations, Genetic
D003546	Cysteine Endopeptidases	ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005798	Genes, Bacterial	The functional hereditary units of BACTERIA.	Bacterial Gene,Bacterial Genes,Gene, Bacterial
D006384	Hemagglutination	The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).	Hemagglutinations
D006388	Hemagglutinins	Agents that cause agglutination of red blood cells. They include antibodies, blood group antigens, lectins, autoimmune factors, bacterial, viral, or parasitic blood agglutinins, etc.	Isohemagglutinins,Exohemagglutinins,Hemagglutinin
D000080867	Gingipain Cysteine Endopeptidases	Cysteine endoproteinases, from periodontal pathogen PORPHYROMONAS GINGIVALIS, acting as virulence factors associated with PERIODONTITIS. They are produced as pre-proproteins which mature into ARGININE and LYSINE specific endopeptidases.	Arg-Gingipain,Arginine Gingipain,Gingipain,Gingipain K,Gingipain Proteases,KGP Protease,Lys-Gingipain,Lysine Gingipain,RGP-2 Gingipain,RGPB Protein,Argingipain,Gingipain R,Gingipain R1,Gingipain R2,Gingipains,Arg Gingipain,Cysteine Endopeptidases, Gingipain,Gingipain, Arginine,Gingipain, Lysine,Gingipain, RGP-2,Lys Gingipain,RGP 2 Gingipain
D001426	Bacterial Proteins	Proteins found in any species of bacterium.	Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial