BIOMAT Database Logo BIOMAT Database Logo

Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry. 1996

T V Pestova, and C U Hellen, and I N Shatsky
Department of Microbiology and Immunology, Morse Institute for Molecular Genetics, State University of New York Health Science Center at Brooklyn, 11203-2098, USA.

Translation of picornavirus RNA is initiated after ribosomal binding to an internal ribosomal entry site (IRES) within the 5' untranslated region. We have reconstituted IRES-mediated initiation on encephalomyocarditis virus RNA from purified components and used primer extension analysis to confirm the fidelity of 48S preinitiation complex formation. Eukaryotic initiation factor 2 (eIF2), eIF3, and eIF4F were required for initiation; eIF4B and to a lesser extent the pyrimidine tract-binding protein stimulated this process. We show that eIF4F binds to the IRES in a novel cap-independent manner and suggest that cap- and IRES-dependent initiation mechanisms utilize different modes of interaction with this factor to promote ribosomal attachment to mRNA.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010448 Peptide Initiation Factors Protein factors uniquely required during the initiation phase of protein synthesis in GENETIC TRANSLATION. Initiation Factors,Initiation Factor,Factors, Peptide Initiation,Initiation Factors, Peptide
D010849 Picornaviridae A family of small RNA viruses comprising some important pathogens of humans and animals. Transmission usually occurs mechanically. There are nine genera: APHTHOVIRUS; CARDIOVIRUS; ENTEROVIRUS; ERBOVIRUS; HEPATOVIRUS; KOBUVIRUS; PARECHOVIRUS; RHINOVIRUS; and TESCHOVIRUS. Avihepatovirus,Pasivirus,Picornaviruses,Rosavirus,Sapelovirus,Senecavirus,Sicinivirus,Tremovirus,Avihepatoviruses,Pasiviruses,Rosaviruses,Sapeloviruses,Senecaviruses,Siciniviruses,Tremoviruses
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012270 Ribosomes Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION. Ribosome
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D012367 RNA, Viral Ribonucleic acid that makes up the genetic material of viruses. Viral RNA
D015852 Eukaryotic Initiation Factor-2 Eukaryotic initiation factor of protein synthesis. In higher eukaryotes the factor consists of three subunits: alpha, beta, and gamma. As initiation proceeds, eIF-2 forms a ternary complex with Met-tRNAi and GTP. EIF-2,Peptide Initiation Factor EIF-2,EIF-2 alpha,EIF-2 beta,EIF-2 gamma,EIF-2alpha,EIF-2beta,EIF-2gamma,EIF2,Eukaryotic Initiation Factor-2, alpha Subunit,Eukaryotic Initiation Factor-2, beta Subunit,Eukaryotic Initiation Factor-2, gamma Subunit,Eukaryotic Peptide Initiation Factor-2,EIF 2,EIF 2 alpha,EIF 2 beta,EIF 2 gamma,EIF 2alpha,EIF 2beta,EIF 2gamma,Eukaryotic Initiation Factor 2,Eukaryotic Initiation Factor 2, alpha Subunit,Eukaryotic Initiation Factor 2, beta Subunit,Eukaryotic Initiation Factor 2, gamma Subunit,Eukaryotic Peptide Initiation Factor 2,Initiation Factor-2, Eukaryotic,Peptide Initiation Factor EIF 2
D039562 Eukaryotic Initiation Factor-4F A trimeric peptide initiation factor complex that associates with the 5' MRNA cap structure of RNA (RNA CAPS) and plays an essential role in MRNA TRANSLATION. It is composed of EUKARYOTIC INITIATION FACTOR-4A; EUKARYOTIC INITIATION FACTOR-4E; and EUKARYOTIC INITIATION FACTOR-4G. EIF-4F,Peptide Initiation Factor EIF-4F,EIF-(iso)4F,EIF4F,EIF4F Translation Initiation Complex,Eukaryotic Initiation Factor-(iso)4F,Protein Initiation Factor EIF-4F,EIF 4F,Eukaryotic Initiation Factor 4F,Initiation Factor-4F, Eukaryotic,Peptide Initiation Factor EIF 4F,Protein Initiation Factor EIF 4F

Related Publications

T V Pestova, and C U Hellen, and I N Shatsky
Translation initiation by factor-independent binding of eukaryotic ribosomes to internal ribosomal entry sites.
July 2005, Comptes rendus biologies,
T V Pestova, and C U Hellen, and I N Shatsky
Eukaryotic translation initiation factor 4E availability controls the switch between cap-dependent and internal ribosomal entry site-mediated translation.
December 2005, Molecular and cellular biology,
T V Pestova, and C U Hellen, and I N Shatsky
Regulation of internal ribosomal entry site-mediated translation by phosphorylation of the translation initiation factor eIF2alpha.
May 2002, The Journal of biological chemistry,
T V Pestova, and C U Hellen, and I N Shatsky
Internal translation initiation and eIF4F/ATP-independent scanning of mRNA by eukaryotic ribosomal particles.
March 2014, Scientific reports,
T V Pestova, and C U Hellen, and I N Shatsky
Non-Canonical Translation Initiation Mechanisms Employed by Eukaryotic Viral mRNAs.
September 2021, Biochemistry. Biokhimiia,
T V Pestova, and C U Hellen, and I N Shatsky
Eukaryotic translation initiation factor 4GI and p97 promote cellular internal ribosome entry sequence-driven translation.
September 2005, Proceedings of the National Academy of Sciences of the United States of America,
T V Pestova, and C U Hellen, and I N Shatsky
Pestivirus translation initiation occurs by internal ribosome entry.
January 1995, Virology,
T V Pestova, and C U Hellen, and I N Shatsky
Translation of encephalomyocarditis virus RNA by internal ribosomal entry.
January 1995, Current topics in microbiology and immunology,
T V Pestova, and C U Hellen, and I N Shatsky
Translation initiation of the human tau mRNA through an internal ribosomal entry site.
January 2009, Journal of Alzheimer's disease : JAD,
T V Pestova, and C U Hellen, and I N Shatsky
Circularization of mRNA by eukaryotic translation initiation factors.
July 1998, Molecular cell,
Copied contents to your clipboard!