-transparent.png){kind=logo}
The acute-phase response is believed to be an important systemic defence reaction to inflammation during infection, trauma, injury or neoplasia. Although the interleukin-6 (IL-6) family of cytokines appear to be the major regulators of the acute-phase reaction, the exact biological significance of this process remains unknown. In this study, a panel of monoclonal antibodies (Mabs) was raised against the extracellular domain of human gp130 (the common signal transducing chain of the IL-6 cytokine family) in order to inhibit the biological activity of IL-6-like cytokines in vivo. Mabs designated 4B11 and 2H4 were most effective in the inhibition of the in vitro acute-phase response on hepatoma cells and prevented the IL-6-induced growth inhibition of A375 cells. Administration of the antibodies to dogs at a dosage of 8 mg/kg/d showed that 2H4 was a potent inhibitor of the IL-6-induced (40 micrograms/kg/d) acute-phase response, abrogating IL-6-mediated increments in fibrinogen, C-reactive protein and the platelet count. This antibody, the first described to abrogate the acute-phase response in vivo, may not only permit development of a new anti-inflammatory strategy, but provides an excellent tool for defining the function of acute-phase proteins in inflammation and infection.
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
March 1997,
European journal of immunology,
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
April 1997,
Cytokine,
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
January 1988,
Annual review of medicine,
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
January 1983,
Journal of immunology (Baltimore, Md. : 1950),
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
July 2002,
FEBS letters,
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
August 1985,
Molecular and cellular biology,
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
April 1986,
Cancer research,
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
March 1990,
FEBS letters,
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
November 1997,
Biochemistry and molecular biology international,
P Harrison, and
T Downs, and
P Friese, and
R Wolf, and
J N George, and
S A Burstein
February 1995,
Immunology letters,
