Biomaterial Database

GLC determination of free and conjugated triclosan in human plasma and urine. 1977

A Sioufi, and F Caudal, and D Grimault

A method for the determination of free and conjugated triclosan at concentrations as low as 2 ng/ml in human plasma or urine is described. Conjugated metabolites are split by enzyme hydrolysis. After addition of an internal standard, triclosan is extracted at acid pH into petroleum ether, transferred to an alkaline aqueous solution, and back-estracted into petroleum ether after acidification. Both compounds are acetylated with acetic anhydride in the presence of pyridine. The acetyl derivatives are determined by GLC using a 63Ni-electron-capture detector.

UI	MeSH Term	Description	Entries
D010647	Phenyl Ethers	Ethers that are linked to a benzene ring structure.	Diphenyl Oxide,Diphenyl Oxides,Diphenyl Ethers,Ethers, Diphenyl,Ethers, Phenyl,Oxide, Diphenyl,Oxides, Diphenyl
D002849	Chromatography, Gas	Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.	Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D005966	Glucuronidase		Endo-beta-D-Glucuronidase,Endoglucuronidase,Exo-beta-D-Glucuronidase,beta-Glucuronidase,Endo beta D Glucuronidase,Exo beta D Glucuronidase,beta Glucuronidase
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006868	Hydrolysis	The process of cleaving a chemical compound by the addition of a molecule of water.
D000107	Acetylation	Formation of an acetyl derivative. (Stedman, 25th ed)	Acetylations
D013429	Sulfatases	A class of enzymes that catalyze the hydrolysis of sulfate ESTERS.	Sulfatase
D014260	Triclosan	A diphenyl ether derivative used in cosmetics and toilet soaps as an antiseptic. It has some bacteriostatic and fungistatic action.	2,4,4'-Trichloro-2'-Hydroxydiphenyl Ether,2-Hydroxy-2',4,4'-trichlorodiphenyl Ether,Aquasept,Clearasil Daily Face Wash,Cliniclean,Irgasan DP 300,Irgasan DP300,Irgasan-DP300,Manusept,Microshield T,Oxy Skin Wash,Sapoderm,Ster-Zac Bath Concentrate,Tersaseptic,Trisan,pHisoHex,DP300, Irgasan,Ster Zac Bath Concentrate,SterZac Bath Concentrate