-transparent.png){kind=logo}
Altered genomic methylcytosine content has been described for a number of tumor types, including neuroblastoma. However, it remains to be determined for different tumor types whether specific loci or chromosomal regions are affected by a methylation change or whether the change is random. We have implemented a computer-based approach for the analysis of two-dimensional separations of human genomic restriction fragments. Through the use of methylation-sensitive restriction enzymes, methylation differences in genomic DNA between tumor and normal tissues can be detected. We report the cloning and sequencing of two fragments detectable in two-dimensional separations of genomic DNA of neuroblastomas. These fragments were found to be a part of repetitive units that exhibited demethylation in neuroblastoma relative to other tumor types. Our finding of a distinct pattern of methylation of repetitive units in neuroblastoma suggests that altered methylation at certain loci may contribute to the biology of this tumor.
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
January 1986,
Cold Spring Harbor symposia on quantitative biology,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
February 1995,
Electrophoresis,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
January 2002,
Oncogene,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
November 2019,
Genes,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
September 1988,
Nucleic acids research,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
August 1993,
International journal of oncology,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
January 1972,
International review of cytology,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
May 2001,
Current protocols in human genetics,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
August 1992,
Clinica chimica acta; international journal of clinical chemistry,
D Thoraval, and
J Asakawa, and
K Wimmer, and
R Kuick, and
B Lamb, and
B Richardson, and
P Ambros, and
T Glover, and
S Hanash
December 1992,
Journal of the International Federation of Clinical Chemistry,
