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In order to precisely define human lymphocytes subsets, we used four color immunofluorescence analysis and flow cytometry. We report here the results of systematic studies performed on whole blood from 20 normal volunteers. This was performed using monoclonal antibodies recognizing 18 different CD molecules and analyzed here into 14 different 4-color immunofluorescence combinations. This showed that among T cells, the two major CD3+CD8+ and CD3+CD8- subsets differed greatly in the percentage of cells expressing CD26, CD27, CD28, CD38, CD45RA, CD45RO, CD57, S6F1, BY55, CD101 and their respective combination representing an array of subsets. Furthermore the minor NK subsets, i.e. CD2+CD3-CD8+, CD2-CD3-CD8+, CD2+CD3-CD8-, CD2-CD3-CD8- also differed in percentages of cells expressing CD16, CD56, CD57, S6F1, BY55 and their combinations.
V Schiavon, and
P Roth, and
W E Bolton, and
J P Farcet, and
A Bensussan, and
L Boumsell
October 2018,
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V Schiavon, and
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J P Farcet, and
A Bensussan, and
L Boumsell
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L Boumsell
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A Bensussan, and
L Boumsell
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A Bensussan, and
L Boumsell
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V Schiavon, and
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J P Farcet, and
A Bensussan, and
L Boumsell
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V Schiavon, and
P Roth, and
W E Bolton, and
J P Farcet, and
A Bensussan, and
L Boumsell
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V Schiavon, and
P Roth, and
W E Bolton, and
J P Farcet, and
A Bensussan, and
L Boumsell
April 1987,
Journal of immunological methods,
