Biomaterial Database

Kinetic analysis of modification reactions at comparable enzyme and modifier concentrations. 1996

K Y Zhao, and Z X Wang

Institute of Biophysics, Academia Sinica, Beijing, China.

A method is proposed to determine the kinetic constants of enzyme modification where the condition of [Y0] >> [E0] is not satisfied, which is applicable to both inhibition and activation. This is a simple and rigorous one by which the apparent rate constants can be conveniently calculated, provided that the values of vo, vs, v* and [P]* are experimentally measured. The equations derived for the calculation of the apparent rate constants can be reduced to those obtained when [Y0] >> [E0]. Based on the expressions of the apparent rate constant and the apparent association constant, similar plotting methods can be applied to distinguish between the different competition types for both reversible and irreversible inhibitions.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008954	Models, Biological	Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.	Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D004789	Enzyme Activation	Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.	Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D004791	Enzyme Inhibitors	Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.	Enzyme Inhibitor,Inhibitor, Enzyme,Inhibitors, Enzyme
D004798	Enzymes	Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.	Biocatalyst,Enzyme,Biocatalysts
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia