Biomaterial Database

Leucocyte-endothelial interactions in chronic critical limb ischaemia. 1996

G Ciuffetti, and R Lombardini, and L Pasqualini, and G Vaudo

Sezione di Medicina Interna, Angiologia e Malattie da Arteriosclerosi, Università degli Studi di Perugia.

Forearm blood samples were drawn from 18 patients with critical limb ischaemia (stage IIIb peripheral arterial obliterative disease) and 18 age- and sex-matched controls at 8.00 a.m., 2.00 p.m. and 8.00 p.m. We monitored the total and differential leucocyte counts and leucocyte expression of CD11/CD18 integrins (on whole blood samples by indirect immunofluorescence on a FACScan flow cytometer). Our results show no significant difference in any parameter at any stage of the day. At all study times, the total and differential leucocyte counts and granulocyte expression of CD11/CD18 were significantly (p < 0.001) greater in patients compared with controls. Associated with a high leucocyte count, constant up-regulation appears to be a feature of chronic tissue ischaemia. Activated neutrophils seem to aggravate peripheral arterial obliterative disease locally and may contribute to disseminated organ damage.

UI	MeSH Term	Description	Entries
D007511	Ischemia	A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.	Ischemias
D007866	Leg	The inferior part of the lower extremity between the KNEE and the ANKLE.	Legs
D007958	Leukocyte Count	The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.	Blood Cell Count, White,Differential Leukocyte Count,Leukocyte Count, Differential,Leukocyte Number,White Blood Cell Count,Count, Differential Leukocyte,Count, Leukocyte,Counts, Differential Leukocyte,Counts, Leukocyte,Differential Leukocyte Counts,Leukocyte Counts,Leukocyte Counts, Differential,Leukocyte Numbers,Number, Leukocyte,Numbers, Leukocyte
D007962	Leukocytes	White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).	Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D008297	Male		Males
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D003627	Data Interpretation, Statistical	Application of statistical procedures to analyze specific observed or assumed facts from a particular study.	Data Analysis, Statistical,Data Interpretations, Statistical,Interpretation, Statistical Data,Statistical Data Analysis,Statistical Data Interpretation,Analyses, Statistical Data,Analysis, Statistical Data,Data Analyses, Statistical,Interpretations, Statistical Data,Statistical Data Analyses,Statistical Data Interpretations
D004730	Endothelium, Vascular	Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.	Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man