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An effective resolution of intact phosphatidylserines on the basis of unsaturation has been achieved by conventional argentation thin layer chromatography (TLC) following trifluoracetylaction. The trifluoroacetamides are prepared by treatment with trifluoroacetic anhydride or N-methyl-bis-trifluoroacetamide. The acetamides are resolved with chloroform-methanol-water (65:25:4, v/v/v) on Silica Gel G containing 20% silver nitrate. Subfractions with 0-6 double bonds per molecule were obtained for the phosphatidylserines of pig and ox brain, pig erythrocytes, rat liver, and rabbit skeletal muscle. The preparation of trifluoroacetamides is also advantageous for the silver ion fractionation of phosphatidylethanolamines. The method is applicable to metabolic studies of molecular species using radioactive precursors of neutral lipids, phosphorus, and nitrogenous bases.
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
July 1971,
Journal of lipid research,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
May 1968,
Journal of chromatography,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
November 1971,
Doklady Akademii nauk SSSR,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
January 1954,
Mitteilungen aus dem Gebiete der Lebensmittel-untersuchung un Hygiene = Travaux de chimie alimentaire et d'hygiene,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
November 1987,
Journal of chromatography,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
June 1968,
Chemistry and physics of lipids,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
April 1968,
Journal of chromatography,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
April 1964,
Journal of lipid research,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
February 1963,
Analytical biochemistry,
S K Yeung, and
A Kuksis, and
L Marai, and
J J Myher
March 1975,
The Biochemical journal,
