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A gene encoding a variant of green fluorescent protein (GFP) of Aequorea victoria was put under the control of a promoter which is constitutive in Rhizobium meliloti. The heterologous GFP gene was expressed at high levels during all stages of symbiosis, allowing R. meliloti cells to be visualized as they grew in the rhizosphere, on the root surface, and inside infection threads. In addition, nodules that were infected with bacteria which were synthesizing GFP fluoresced when illuminated with blue light. GFP-tagged bacteria could be seen inside infection threads, providing the opportunity to measure the growth rate and determine the patterns of growth of R. meliloti residing inside its host plant.
D J Gage, and
T Bobo, and
S R Long
January 2006,
Mikrobiologiia,
D J Gage, and
T Bobo, and
S R Long
August 1998,
Proceedings of the National Academy of Sciences of the United States of America,
D J Gage, and
T Bobo, and
S R Long
March 1993,
Plant physiology,
D J Gage, and
T Bobo, and
S R Long
October 1977,
Archives of microbiology,
D J Gage, and
T Bobo, and
S R Long
January 2019,
Frontiers in plant science,
D J Gage, and
T Bobo, and
S R Long
April 2004,
Genetika,
D J Gage, and
T Bobo, and
S R Long
September 2002,
Indian journal of experimental biology,
D J Gage, and
T Bobo, and
S R Long
June 2007,
Ying yong sheng tai xue bao = The journal of applied ecology,
D J Gage, and
T Bobo, and
S R Long
August 2022,
Environmental microbiology reports,
D J Gage, and
T Bobo, and
S R Long
December 1984,
Applied and environmental microbiology,
