Biomaterial Database

Effect of polyionic compounds on the adsorption of polyoma virus. 1996

Y W Yang, and J C Yang

School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan.

The inhibitory effect of polyionic compounds, including dextran sulfates and poly-L-lysines of various molecular weight, on the cytopathogenicity of polyoma virus was studied. Poly-L-lysines were found to be more potent inhibitors of polyoma virus than dextran sulfates in UC1B cells. The 50% effective concentrations (EC50) were found to be inversely related to the molecular weight of the polymers. The higher the molecular weight of poly-L-lysine, the more effective was the polymer in inhibiting virus-induced cytopathogenicity. Incubation of the cells with poly-L-lysines was found to increase the zeta potential of the cells, whereas no significant change of the electrokinetic behavior was observed for dextran sulfate-treated cells, indicating that poly-L-lysines were adsorbed to the cells and prevented virus adsorption through steric hindrance effect. Studies using 3H-labeled virions confirmed that poly-L-lysines inhibit virus adsorption to the host cells. The results obtained from this study indicated that poly-L-lysines bind preferentially to UC1B cells through electrostatic interactions, whereas dextran sulfates are not effective inhibitors of polyoma virus.

UI	MeSH Term	Description	Entries
D011107	Polylysine	A peptide which is a homopolymer of lysine.	Epsilon-Polylysine,Poly-(Alpha-L-Lysine),Epsilon Polylysine
D011120	Polyomavirus	A genus of potentially oncogenic viruses of the family POLYOMAVIRIDAE. These viruses are normally present in their natural hosts as latent infections. The virus is oncogenic in hosts different from the species of origin.	Bovine polyomavirus,Murine polyomavirus,Hamster polyomavirus,Polyoma Virus,Polyoma Viruses,Bovine polyomaviruses,Hamster polyomaviruses,Murine polyomaviruses,Polyomaviruses,Virus, Polyoma,Viruses, Polyoma,polyomavirus, Hamster,polyomaviruses, Bovine,polyomaviruses, Murine
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016264	Dextran Sulfate	Long-chain polymer of glucose containing 17-20% sulfur. It has been used as an anticoagulant and also has been shown to inhibit the binding of HIV-1 to CD4-POSITIVE T-LYMPHOCYTES. It is commonly used as both an experimental and clinical laboratory reagent and has been investigated for use as an antiviral agent, in the treatment of hypolipidemia, and for the prevention of free radical damage, among other applications.	Sodium Dextran Sulfate,Dextran Sulfate Sodium,Dextran Sulfate, Sodium,Sulfate Sodium, Dextran,Sulfate, Dextran,Sulfate, Sodium Dextran
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus