BIOMAT Database Logo BIOMAT Database Logo

Validation of the Applied Biosystems Prism 377 automated sequencer for the forensic short tandem repeat analysis. 1996

R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Forensic Science Service, Birmingham, UK.

The Applied Biosystems (ABI) Prism 377 DNA sequencer has been evaluated in an attempt to increase the throughput of samples for short tandem repeat (STR) analysis, in both forensic casework and the UK National Criminal Intelligence DNA Database. The gel system assessed consisted of 0.2 mm, 4% acrylamide 6 M urea gels, with a well-to-read distance of 36 cm. Gels were run at a constant voltage of 3 kV and constant temperature of 51 degrees C. The run time of our second generation multiplex (SGM) STR system was achieved in less than 2 h. Rigorous validation has been performed on the instrument hardware and software. Complete resolution of 1 base differences was obtained, up to and beyond 350 bases; sizing precision across gels was more than 2-fold higher than the 373A and the sensitivity was increased by one third.

UI MeSH Term Description Entries
D012091 Repetitive Sequences, Nucleic Acid Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES). DNA Repetitious Region,Direct Repeat,Genes, Selfish,Nucleic Acid Repetitive Sequences,Repetitive Region,Selfish DNA,Selfish Genes,DNA, Selfish,Repetitious Region, DNA,Repetitive Sequence,DNA Repetitious Regions,DNAs, Selfish,Direct Repeats,Gene, Selfish,Repeat, Direct,Repeats, Direct,Repetitious Regions, DNA,Repetitive Regions,Repetitive Sequences,Selfish DNAs,Selfish Gene
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D005554 Forensic Medicine The application of medical knowledge to questions of law. Legal Medicine,Medicine, Forensic,Medicine, Legal
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001322 Autoanalysis Method of analyzing chemicals using automation. Autoanalyses
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA

Related Publications

R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Sequencing reactions for the applied biosystems 373A Automated DNA Sequencer.
January 1993, Methods in molecular biology (Clifton, N.J.),
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
A fully integrated microchip system for automated forensic short tandem repeat analysis.
May 2017, The Analyst,
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Microsatellite fragment analysis using the ABI PRISM ® 377 DNA sequencer.
January 2013, Methods in molecular biology (Clifton, N.J.),
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Validation of the HumDXS6807 short tandem repeat polymorphism for forensic application.
October 1999, Electrophoresis,
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Automated short tandem repeat (STR) analysis in forensic casework--a strategy for the future.
September 1995, Electrophoresis,
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Quantitative analysis of chimerism using a short tandem repeat method on a fluorescent automated DNA sequencer.
February 2006, Clinical and laboratory haematology,
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Increased throughput for fragment analysis on an ABI PRISM 377 automated sequencer using a membrane comb and STRand software.
December 2001, BioTechniques,
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Forensic validation of the short tandem repeat HUMACTBP2 using capillary electrophoresis.
February 1998, Electrophoresis,
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
Optimization of Applied Biosystems 373 sequencer stretch modification.
September 1995, BioTechniques,
R R Frazier, and E S Millican, and S K Watson, and N J Oldroyd, and R L Sparkles, and K M Taylor, and S Panchal, and L Bark, and C P Kimpton, and P D Gill
The validation of short tandem repeat (STR) loci for use in forensic casework.
January 1994, International journal of legal medicine,
Copied contents to your clipboard!