Biomaterial Database

The chick embryo chorioallantoic membrane as an in vivo wound healing model. 1996

D Ribatti, and A Vacca, and G Ranieri, and S Sorino, and L Roncali

Institute of Human Anatomy, Histology and Embryology, University of Bari Medical School, Italy.

The chick embryo chorioallantoic membrane (CAM) was used as an in vivo wound healing model. A full excision of a 1 mm2 CAM area was filled by a granulation tissue after 96-120 h, which eventually formed a scar in 75% of the cases. In the remaining 25%, a solution of continuity was left which, however, was smaller in size than the one observed immediately after the excision. Under the microscope, the CAM area involved in the repair process showed: i. hyperplasia of the chorionic epithelium; ii. about three times as many microvessels and fibroblasts in the mesenchyme as in the normal adjacent control regions; iii. an inflammatory infiltrate mostly consisting of macrophages; and iv. a strong positivity for fibronectin in the extracellular matrix. The validity of this experimental model appears to be confirmed by the fact that we were able to reproduce all the critical events controlling the wound healing process, such as re-epithelization, angiogenesis, formation of an inflammatory infiltrate and deposition of one of the main constituents of the extracellular matrix, such as fibronectin.

UI	MeSH Term	Description	Entries
D008954	Models, Biological	Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.	Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D002642	Chick Embryo	The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.	Embryo, Chick,Chick Embryos,Embryos, Chick
D002823	Chorion	The outermost extra-embryonic membrane surrounding the developing embryo. In REPTILES and BIRDS, it adheres to the shell and allows exchange of gases between the egg and its environment. In MAMMALS, the chorion evolves into the fetal contribution of the PLACENTA.	Chorions
D000482	Allantois	An extra-embryonic membranous sac derived from the YOLK SAC of REPTILES; BIRDS; and MAMMALS. It lies between two other extra-embryonic membranes, the AMNION and the CHORION. The allantois serves to store urinary wastes and mediate exchange of gas and nutrients for the developing embryo.	Allantoic Membrane,Membrane, Allantoic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014945	Wound Healing	Restoration of integrity to traumatized tissue.	Healing, Wound,Healings, Wound,Wound Healings