BIOMAT Database Logo BIOMAT Database Logo

Gamma radiation effect on vascular smooth muscle cells in culture. 1996

C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Department of Neurological Surgery, University of Washington, Seattle 98108, USA.

OBJECTIVE Prior work in our laboratory demonstrated that external gamma irradiation administered within 48 h following balloon catheter carotid artery injury in rats produced a marked inhibition of intimal hyperplasia and restenosis. The current study used smooth muscle cells (SMC) in vitro to examine the radiation dose response and to investigate the cellular mechanism by which radiation inhibits SMC proliferation. METHODS Quiescent rat aortic SMC in plasma were refed with whole blood serum to stimulate synchronous proliferation and immediately irradiated with single fraction doses of 1.25-20 Gy. RESULTS Comparison between a micronucleus assay and a clonogenic assay indicated a dose-dependent inhibition of SMC growth, with an ED50 at 2-3 Gy. The micronucleus assay also demonstrated a dose-dependent increase in apparent chromosomal damage at 72 h after irradiation. Inhibition of SMC growth by radiation did not correlate with changes in intracellular or released mitogenic activity. Furthermore, there was no evidence of apoptosis in irradiated SMC up to 96 h after treatment. CONCLUSIONS Radiation likely inhibits SMC proliferation after arterial injury by a dose-dependent mechanism of lethal and/or sublethal cellular injury leading to clonogenic cell death.

UI MeSH Term Description Entries
D008934 Mitogens Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed) Mitogen,Phytomitogen,Phytomitogens
D009131 Muscle, Smooth, Vascular The nonstriated involuntary muscle tissue of blood vessels. Vascular Smooth Muscle,Muscle, Vascular Smooth,Muscles, Vascular Smooth,Smooth Muscle, Vascular,Smooth Muscles, Vascular,Vascular Smooth Muscles
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D003114 Colony-Forming Units Assay A cytologic technique for measuring the functional capacity of stem cells by assaying their activity. Clonogenic Cell Assay,Stem Cell Assay,Clonogenic Cell Assays,Colony Forming Units Assays,Colony-Forming Units Assays,Stem Cell Assays,Assay, Clonogenic Cell,Assay, Colony-Forming Units,Assay, Stem Cell,Assays, Clonogenic Cell,Assays, Colony-Forming Units,Assays, Stem Cell,Colony Forming Units Assay
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004247 DNA A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004307 Dose-Response Relationship, Radiation The relationship between the dose of administered radiation and the response of the organism or tissue to the radiation. Dose Response Relationship, Radiation,Dose-Response Relationships, Radiation,Radiation Dose-Response Relationship,Radiation Dose-Response Relationships,Relationship, Radiation Dose-Response,Relationships, Radiation Dose-Response
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015162 Micronucleus Tests Induction and quantitative measurement of chromosomal damage leading to the formation of micronuclei (MICRONUCLEI, CHROMOSOME-DEFECTIVE) in cells which have been exposed to genotoxic agents or IONIZING RADIATION. Micronucleus Assays,Assay, Micronucleus,Assays, Micronucleus,Micronucleus Assay,Micronucleus Test,Test, Micronucleus,Tests, Micronucleus
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis

Related Publications

C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Stimulatory effect of serum on 86Rb washout from vascular smooth muscle cells in culture.
January 1990, Pharmacological research,
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Vascular smooth muscle cells: isolation, culture, and characterization.
January 2012, Methods in molecular biology (Clifton, N.J.),
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Vascular smooth muscle cells : isolation, culture, and characterization.
January 2001, Methods in molecular medicine,
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Effect of cyclosporin on vascular smooth muscle cells.
July 1991, Laboratory investigation; a journal of technical methods and pathology,
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Gbeta gamma mediate differentiation of vascular smooth muscle cells.
June 2001, The Journal of biological chemistry,
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Specific binding of endothelin on human vascular smooth muscle cells in culture.
May 1989, The Journal of clinical investigation,
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Isolation, Culture, and Characterization of Vascular Smooth Muscle Cells.
January 2016, Methods in molecular biology (Clifton, N.J.),
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Isolation, culture and characterisation of vascular smooth muscle cells.
January 2009, Methods in molecular biology (Clifton, N.J.),
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Effect of contrast media on vascular smooth muscle cells.
October 2004, Acta radiologica (Stockholm, Sweden : 1987),
C M Gajdusek, and H Tian, and S London, and D Zhou, and J Rasey, and M R Mayberg
Effect of centrifugal force and catecholamines on glycosaminoglycans synthesis of vascular smooth muscle cells in culture.
August 1990, Atherosclerosis,
Copied contents to your clipboard!