Biomaterial Database

[Comparison of 2 serologic methods for the diagnosis of hydatidosis]. 1996

H M Arienti, and S I Guignard, and D E Rinaldi, and O C Elbarcha

Laboratorio de Parasitología, Hospital Rawson, Córdoba, Argentina.

The sera of 176 patients with epidemiologic antecedents or radiologic and clinical signs of hydatidosis were tested by counter-immunoelectrophoresis (CIE) and enzyme-linked immunoassay (ELISA). A semipurified antigen from cysts of human origin were used for both techniques. The results were compared with those obtained from complementary radiologic studies and were confirmed by examination of excised cysts. Biopsy confirmed the diagnosis of hydatidosis in 65 patients (37%) and revealed the presence of other diseases in the remaining 111 (63%). Of the original 176 patients, 36 (20.4%) were positive by CIE and 62 (35.2%) by ELISA. Both techniques showed an excellent correlation with postsurgical diagnosis; neither produced any false positives, and the ELISA gave false negative results for only three patients (4.6%) with cysts that were infected, infertile, or calcified to some degree. The paper describes standardization of an inexpensive and easy-to-use microELISA.

UI	MeSH Term	Description	Entries
D003378	Counterimmunoelectrophoresis	Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.	Electrosyneresis,Immunoelectroosmophoresis,Immunoelectrophoresis, Countercurrent,Immunoelectrophoresis, Crossover,Countercurrent Immunoelectrophoresis,Crossover Immunoelectrophoresis
D004443	Echinococcosis	An infection caused by the infestation of the larval form of tapeworms of the genus Echinococcus. The liver, lungs, and kidney are the most common areas of infestation.	Cystic Echinococcosis,Cysts, Hydatid,Hydatid Cyst,Hydatidosis,Echinococcus Granulosus Infection,Echinococcus Infection,Hydatid Disease,Cyst, Hydatid,Cystic Echinococcoses,Echinococcoses,Echinococcoses, Cystic,Echinococcosis, Cystic,Echinococcus Granulosus Infections,Echinococcus Infections,Granulosus Infection, Echinococcus,Granulosus Infections, Echinococcus,Hydatid Cysts,Hydatid Diseases,Hydatidoses,Infection, Echinococcus,Infection, Echinococcus Granulosus,Infections, Echinococcus Granulosus
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000909	Antibodies, Helminth	Immunoglobulins produced in a response to HELMINTH ANTIGENS.	Helminth Antibodies
D000947	Antigens, Helminth	Any part or derivative of a helminth that elicits an immune reaction. The most commonly seen helminth antigens are those of the schistosomes.	Helminth Antigens