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The effects of mechanical strain on monocyte chemotactic protein-1 (MCP-1) secretion were examined on human endothelial cells (ECs) grown on a flexible membrane base. MCP-1 release into culture medium from strained ECs was demonstrated to be time and strain dose dependent. Northern blot analysis demonstrated a mainly serum-independent 1.8-fold induction of MCP-1 mRNA levels in ECs strained at 15 kPa compared with unstrained controls. ECs treated with actinomycin D abolished this strain-induced expression. Strained ECs at the periphery of wells showed higher MCP-1 gene expression than ECs at the center. Pretreatment of ECs with either cytochalasin D or phalloidin did not abolish strain-induced gene expression. ECs pretreated with stretch-activated ion channel blocker gadolinium or with ryanodine to deplete intracellular stored Ca2+ strongly inhibited the strain-induced MCP-1 levels. We conclude that 1) cyclical strain can modulate the secretion of MCP-1 in a dose-dependent manner, 2) strain-induced MCP-1 production is mediated by increasing MCP-1 mRNA levels via transcription, 3) cytoskeletal rearrangement is not essential for this strain-induced MCP-1 expression, and 4) both Ca2+ influx via stretch-activated ion channels and intracellular Ca2+ release contribute to the strain-induced effect. Such strain-induced MCP-1 secretion might contribute to the trapping of monocytes in the subendothelial space to initiate atherogenesis.
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
September 1993,
Biochemical and biophysical research communications,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
August 1995,
Circulation research,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
October 2005,
American journal of physiology. Heart and circulatory physiology,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
January 2007,
Journal of vascular research,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
July 2005,
Fertility and sterility,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
September 1997,
European cytokine network,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
September 1998,
Circulation,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
December 1999,
Journal of cellular biochemistry,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
October 2000,
Arteriosclerosis, thrombosis, and vascular biology,
B S Wung, and
J J Cheng, and
Y J Chao, and
J Lin, and
Y J Shyy, and
D L Wang
July 1990,
Proceedings of the National Academy of Sciences of the United States of America,
