Biomaterial Database

Differential distribution of neuropeptide Y Y1 and Y2 receptors in rat and guinea-pig brains. 1997

D R Gehlert, and S L Gackenheimer

CNS Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA.

The distribution of neuropeptide Y Y1 and Y2 receptors has been extensively studied in the rat using selective masking of [125I]peptide YY binding by Y1- and Y2-selective peptides. In the present study, we characterized the binding of the subtype-selective radioligands [125I](Leu31-Pro34)-peptide YY and [125I]peptide YY3-36 to rat and guinea-pig brains and identified differences in Y1 and Y2 distribution. [125I](Leu31Pro34)-peptide YY and [125I]peptide YY3-36 bound to single sites in rat and guinea-pig brains with pharmacologies consistent with the Y1 and Y2 receptors, respectively. Autoradiographic studies were conducted using adjacent sections from rat and guinea-pig brains. Compared to the rat, the distribution of both Y1 and Y2 receptors was markedly different in the guinea-pig. For example, a high level of Y1 binding was detected within the thalamus of the rat while, in the guinea-pig, very little Y1 binding was observed in this region. Y1 binding was very low in the rat hippocampus, while the guinea-pig hippocampus contained high levels of Y1 binding. High levels of both Y1 and Y2 binding were observed in the guinea-pig cerebellum while, in the rat, only low levels of Y2 binding were visible. Therefore, the guinea-pig brain exhibits dramatic differences in the pattern of Y1 and Y2 receptors when compared to the rat. It is likely that Y1 and Y2 receptors perform different roles in the central nervous system of guinea-pigs and rats.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009478	Neuropeptide Y	A 36-amino acid peptide present in many organs and in many sympathetic noradrenergic neurons. It has vasoconstrictor and natriuretic activity and regulates local blood flow, glandular secretion, and smooth muscle activity. The peptide also stimulates feeding and drinking behavior and influences secretion of pituitary hormones.	Neuropeptide Y-Like Immunoreactive Peptide,Neuropeptide Tyrosine,Neuropeptide Y Like Immunoreactive Peptide,Tyrosine, Neuropeptide
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D010455	Peptides	Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are considered to be larger versions of peptides that can form into complex structures such as ENZYMES and RECEPTORS.	Peptide,Polypeptide,Polypeptides
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001345	Autoradiography	The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)	Radioautography
D001665	Binding Sites	The parts of a macromolecule that directly participate in its specific combination with another molecule.	Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D013045	Species Specificity	The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.	Species Specificities,Specificities, Species,Specificity, Species