BIOMAT Database Logo BIOMAT Database Logo

Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application. 1996

S Pongsunk, and P Ekpo, and T Dharakul
Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Hybridomas secreting monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei were produced by immunizing BALB/cJ mice with crude culture filtrate of B. pseudomallei. Two monoclonal antibodies were found to be highly specific to B.pseudomallei as tested by indirect enzyme-linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from B. pseudomallei, B. cepacia, Pseudomonas aeruginosa, P.putida, and Escherichia coli. One of the specific MAbs, clone SP-M, IgM subclass, could directly agglutinate all 42 B. pseudomallei, isolates. The study has shown that the agglutinating MAb has potential for rapid identification of B. pseudomallei in primary bacterial culture from clinical specimens. The antibody can be used in bacteriology laboratories to reduce time of biochemical methods, which require a few days.

UI MeSH Term Description Entries
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000371 Agglutination The clumping together of suspended material resulting from the action of AGGLUTININS.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D016957 Burkholderia pseudomallei A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia. Pseudomonas pseudomallei
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus

Related Publications

S Pongsunk, and P Ekpo, and T Dharakul
Development of mouse hybridomas for production of monoclonal antibodies specific to Burkholderia mallei and Burkholderia pseudomallei.
August 2006, Hybridoma (2005),
S Pongsunk, and P Ekpo, and T Dharakul
Production of Vi monoclonal antibodies and their application as diagnostic reagents.
March 1987, Journal of clinical microbiology,
S Pongsunk, and P Ekpo, and T Dharakul
Polysaccharide specific monoclonal antibodies provide passive protection against intranasal challenge with Burkholderia pseudomallei.
January 2012, PloS one,
S Pongsunk, and P Ekpo, and T Dharakul
Production and application of monoclonal antibodies specific to pyrroloquinoline quinone.
April 1995, Journal of biochemistry,
S Pongsunk, and P Ekpo, and T Dharakul
Immune responses of selected phagotopes from monoclonal antibodies of Burkholderia pseudomallei.
May 2008, The Southeast Asian journal of tropical medicine and public health,
S Pongsunk, and P Ekpo, and T Dharakul
Monoclonal antibodies specific for Shigella dysenteriae serotype 13. Production, characterization, and diagnostic application.
March 1994, Diagnostic microbiology and infectious disease,
S Pongsunk, and P Ekpo, and T Dharakul
Production and characterization of chimeric monoclonal antibodies against Burkholderia pseudomallei and B. mallei using the DHFR expression system.
May 2011, PloS one,
S Pongsunk, and P Ekpo, and T Dharakul
Characterization and potential diagnostic application of monoclonal antibodies specific to rabies virus.
September 2010, Journal of biomedical research,
S Pongsunk, and P Ekpo, and T Dharakul
Development of aptamers specific for potential diagnostic targets in Burkholderia pseudomallei.
December 2008, Transactions of the Royal Society of Tropical Medicine and Hygiene,
S Pongsunk, and P Ekpo, and T Dharakul
Burkholderia pseudomallei antibodies in children, Cambodia.
February 2008, Emerging infectious diseases,
Copied contents to your clipboard!