-transparent.png){kind=logo}
Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects. Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent. Here, we demonstrate by resonance energy transfer (RET) technique that nonspecific, CD14-independent intercalation of LPS into membrane systems can be mediated by lipopolysaccharide-binding protein (LBP). It is proposed that in this pathway, LBP breaks down LPS aggregates, transports the smaller units to and inserts them into the phospholipid cell matrix. We furthermore show that LBP also mediates the intercalation of other negatively charged amphiphilic molecules. We propose a model explaining CD14-independent cell activation at high endotoxin concentrations.
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
April 1997,
Journal of immunology (Baltimore, Md. : 1950),
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
May 1995,
The Journal of biological chemistry,
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
May 2000,
Infection and immunity,
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
February 2006,
The Journal of biological chemistry,
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
January 1994,
The Journal of experimental medicine,
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
March 1994,
Chest,
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
January 1997,
The Journal of clinical investigation,
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
September 1997,
The Journal of biological chemistry,
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
November 1993,
The Journal of biological chemistry,
A B Schromm, and
K Brandenburg, and
E T Rietschel, and
H D Flad, and
S F Carroll, and
U Seydel
April 1993,
Immunobiology,
