Biomaterial Database

Bioprocessing in microgravity: free flow electrophoresis of C. elegans DNA. 1996

H Kobayashi, and N Ishii, and S Nagaoka

Faculty of Science, JOSAI University, Sakado-Shi, Japan.

Free flow electrophoresis of a nematode C. elegans DNA was carried out on the space shuttle flight STS65/Colombia. During the processes of the experiment, the house keeping data of the FFEU and the electrophoretic migration profiles were monitored at POCC (Payload Operations Control Center) of MSFC (Marshall Space Flight Center, Alabama) according to the real-time down-link system. The three dimensional electropherogram (3DEP) on the basis of the down-linked data showed some trouble with the apparatus but three sequential experiments indicated this disturbance of the apparatus is rather preferably stable. Comparing post-flight analyses of the DNA component fractionated, that is, amplification by PCR method, it revealed that the DNAs were separated approximately into two peaks: one of them contained seven-fold higher content of DNA estimated by a sod-4 gene probe than an unc-6 gene probe. These results suggested that this separation technique could be still more effective for the separation of biological macromolecules such as DNA, and the efficiency of separation of the free flow electrophoresis under microgravity environment was useful.

UI	MeSH Term	Description	Entries
D004586	Electrophoresis	An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.	Electrophoreses
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D001709	Biotechnology	Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.	Biotechnologies
D013026	Space Flight	Travel beyond the earth's atmosphere.	Space Exploration,Space Travel,Spaceflight,Exploration, Space,Explorations, Space,Flight, Space,Flights, Space,Space Explorations,Space Flights,Space Travels,Spaceflights,Travel, Space,Travels, Space
D014893	Weightlessness	Condition in which no acceleration, whether due to gravity or any other force, can be detected by an observer within a system. It also means the absence of weight or the absence of the force of gravity acting on a body. Microgravity, gravitational force between 0 and 10 -6 g, is included here. (From NASA Thesaurus, 1988)	Microgravity,Zero Gravity,Gravity, Zero
D017173	Caenorhabditis elegans	A species of nematode that is widely used in biological, biochemical, and genetic studies.	Caenorhabditis elegan,elegan, Caenorhabditis
D017931	DNA Primers	Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.	DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide
D018509	DNA, Helminth	Deoxyribonucleic acid that makes up the genetic material of helminths.	Helminth DNA