TCR in Fas-sensitive T cells from labial salivary glands of patients with Sjögren's syndrome. 1997

T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
Division of Rheumatology and Molecular Immunology, Institute of Medical Science, St. Marianna University, School of Medicine, Kawasaki, Japan.

Apoptosis is found in labial salivary glands of patients with Sjogren's syndrome (SS). To analyze the pathogenesis of apoptosis in labial salivary glands of SS patients, we examined the expression of Fas Ag and Fas ligand (FasL) and TCR on T cells susceptible to anti-Fas mAbs (CH-11). Fas Ag is expressed on epithelial cells and mononuclear cells in the salivary glands as observed by an immunohistochemical method. FasL is over-expressed specifically on T cells infiltrating into the labial salivary glands as seen by an reverse transcription-PCR method. These results suggest that apoptosis in SS lips is mediated by a Fas/FasL pathway. PCR single-strand conformation polymorphism (SSCP) clearly demonstrated that more than 40% of the T cells accumulated in labial salivary glands are deleted by incubation with CH-11 for 24 h in vitro, indicating that these expanded cells are Fas sensitive. junctional sequence analysis showed that the same conserved amino acid motifs (LAGG, RLA, SLG, QGPG, PGG, GGE, RGR, KPG, AGD, and MLG) in complementarity determining region 3 (CDR3) are found in Fas-sensitive T cell clones, whereas they are not detected in Fas-resistant clones, suggesting that Fas-sensitive T cells recognize restricted T cell epitopes on autoantigens. In conclusion, the findings suggest that Fas-sensitive T cells in labial salivary glands of SS patients are generated by Ag stimulation and might function as autoreactive T cells.

UI MeSH Term Description Entries
D008024 Ligands A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed) Ligand
D008046 Lip Either of the two fleshy, full-blooded margins of the mouth. Philtrum,Lips,Philtrums
D008562 Membrane Glycoproteins Glycoproteins found on the membrane or surface of cells. Cell Surface Glycoproteins,Surface Glycoproteins,Cell Surface Glycoprotein,Membrane Glycoprotein,Surface Glycoprotein,Glycoprotein, Cell Surface,Glycoprotein, Membrane,Glycoprotein, Surface,Glycoproteins, Cell Surface,Glycoproteins, Membrane,Glycoproteins, Surface,Surface Glycoprotein, Cell,Surface Glycoproteins, Cell
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012470 Salivary Glands, Minor Accessory salivary glands located in the lip, cheek, tongue, floor of mouth, palate and intramaxillary. Gland, Minor Salivary,Glands, Minor Salivary,Minor Salivary Gland,Minor Salivary Glands,Salivary Gland, Minor
D012859 Sjogren's Syndrome Chronic inflammatory and autoimmune disease in which the salivary and lacrimal glands undergo progressive destruction by lymphocytes and plasma cells resulting in decreased production of saliva and tears. The primary form, often called sicca syndrome, involves both KERATOCONJUNCTIVITIS SICCA and XEROSTOMIA. The secondary form includes, in addition, the presence of a connective tissue disease, usually rheumatoid arthritis. Sicca Syndrome,Sjogren Syndrome,Sjogrens Syndrome,Syndrome, Sicca,Syndrome, Sjogren's
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D016693 Receptors, Antigen, T-Cell, alpha-beta T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules. Antigen Receptors, T-Cell, alpha-beta,T-Cell Receptors alpha-Chain,T-Cell Receptors beta-Chain,T-Cell Receptors, alpha-beta,TcR alpha-beta,Antigen T Cell Receptor, alpha Chain,Antigen T Cell Receptor, beta Chain,Receptors, Antigen, T Cell, alpha beta,T Cell Receptors, alpha beta,T-Cell Receptor alpha-Chain,T-Cell Receptor beta-Chain,T-Cell Receptor, alpha-beta,T Cell Receptor alpha Chain,T Cell Receptor beta Chain,T Cell Receptor, alpha beta,T Cell Receptors alpha Chain,T Cell Receptors beta Chain,TcR alpha beta,alpha-Chain, T-Cell Receptor,alpha-Chain, T-Cell Receptors,alpha-beta T-Cell Receptor,alpha-beta T-Cell Receptors,alpha-beta, TcR,beta-Chain, T-Cell Receptor,beta-Chain, T-Cell Receptors

Related Publications

T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
April 2004, The Journal of pathology,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
September 1994, The Journal of rheumatology,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
November 1995, The Journal of rheumatology,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
January 1992, Autoimmunity,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
August 2012, Clinical and experimental immunology,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
March 1974, The Bulletin of Tokyo Medical and Dental University,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
January 1999, Clinical and experimental rheumatology,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
March 1985, Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
November 1993, Histopathology,
T Sumida, and I Matsumoto, and H Murata, and T Namekawa, and R Matsumura, and H Tomioka, and I Iwamoto, and Y Saito, and Y Mizushima, and T Hasunuma, and T Maeda, and K Nishioka
March 1990, Arthritis and rheumatism,
Copied contents to your clipboard!