Morphology and ultrastructure of Ureaplasma urealyticum in agar growth. 1977

F T Black, and O Vinther

Colonies of Ureaplasma urealyticum serotype VI grown on agar were examined by thin sectioning technique. The arrangement of cells within the colonies was less dense and less stratified than that observed in most other mycoplasma colonies. Small, dense and pleomorphic cells were located mainly along the colony borderline towards the agar. Studies of cell morphology after 3, 10 and 15 days of growth revealed that such cells were younger than the less dense, mostly ovoid cells seen in the central area of colonies. The ultrastructure of individual cells was similar to that of Ureaplasma grown in liquid medium, as described earlier, featuring geometrical ribosome arrangements and extramembranous coat. Serial sections demonstrated the difficulties involved in discussing cell morphology on the basis of single thin sections. The advantages of the preparation method used for electron microscopy are discussed.

UI MeSH Term Description Entries
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D000362 Agar A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D014509 Ureaplasma A genus of gram-negative, nonmotile bacteria which are common parasitic inhabitants of the urogenital tracts of humans, cattle, dogs, and monkeys. T-Mycoplasma,Ureaplasma parvum,Ureaplasma urealyticum biovar 1

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